LOCUS       Exported                 848 bp ds-DNA     linear   SYN 06-JUL-2021
DEFINITION  Mouse Ntaq1-EGFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    pEGFP_mNTAQ1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Wang H, Piatkov KI, Brower CS, Varshavsky A
  TITLE     Glutamine-specific N-terminal amidase, a component of the N-end rule
            pathway.
  JOURNAL   Mol Cell. 2009 Jun 26;34(6):686-95. doi: 
            10.1016/j.molcel.2009.04.032.
  PUBMED    19560421
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      6..15
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        complement(717..816)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(746..766)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctcaccatg gtggcgaccg gtggatcccg ggcccgcggg gcttggtagg ttttgctgct
       61 gaaccgatgc acaaactcag gcagcgtgta gacagctccc catcctactg cagggtccat
      121 gctgatgaag tcgttcaggt tcattttgga gtctccagtt tcaatgcagg ggtactctgg
      181 gggaggctct ctccagttcc cactggagtc tttcatgtga gaccggtcag aagcgaagtg
      241 cttcaagtag gagtcagcac gaaccactct aaatttcctc ctaaactgca gatgaatgtc
      301 atcatcagac ttaagggcat cttctatgta aatgtcgaaa gggcagggaa atggcaaaat
      361 agtgtcaaga tcataaatga agctttgtcc ttcccttgac acatgaagca gaaccacatg
      421 gtagtcccag atcacaggtc cattctcagg tcttgcctgc tgtttccaaa taggtaccat
      481 cttcttctca tttgatatga agactgcata gcactcctcc aacagatact ggttgtgtgt
      541 cttgatgtac tcacagagct tccaaatgtt ttcctcacaa tagcagctgt tgtagacaca
      601 tgcgtcccgc gtaggacaga ccggctggta ctgcggggcc gtggcggcgg ggccgtcccc
      661 ctccatctcg agatctgagt ccggtagcgc tagcggatct gacggttcac taaaccagct
      721 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      781 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      841 tcaatggg
//
