LOCUS Exported 9066 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pFCK(0.4)GW SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9066) AUTHORS Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P TITLE Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. JOURNAL Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11. PUBMED 15608064 REFERENCE 2 (bases 1 to 9066) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9066 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" regulatory 2992..3001 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2998..3717 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 2998..3714 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3043..3064) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3304..3323) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3651..3672 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3758..4346 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3811..3831) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4229..4240) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4349..4365) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4350..4366) /label=pBluescriptKS /note="For pBluescript vector" LTR 4871..5051 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5077..5094) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5083..5307 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5353..5781 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5440..5459) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5650..5671 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5790..5810) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5795..6124 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5975..6110 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6037..6056 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6172..6219 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6238..6612 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 6742..6863 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6779..6798) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6833..6852 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(6912..6928) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6912..6928) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6925..6947) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6936..6952 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6960..6990) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7005..7026 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7143..7160) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7314..7902) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7394..7413) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8073..8933) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8696..8715 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8934..9038) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acttgtggac taagtttgtt cacatcccct tctccaaccc 2641 cctcagtaca tcaccctggg agaacaaggt ccacttgctt ctgggcccac acagtcctgc 2701 agtattgtgt atataaggcc agggcaacgg aggagcaggt tttgaagtga aaggcaggca 2761 ggtgttgggg aggcagttac cggggcaacg ggaacagggc gtttcggagg tggttgccat 2821 ggggacctgg atgctgacga aggctcgcga ggctgtgagc agccacagtg ccctgctcag 2881 aagccccaag ctcgtcaatc aagctggttc tccatttgca ctcaggagca cgggcaggcg 2941 agtggcccct agttctgggg gcagctctag agcgatcccc gggtaccggt cgccaccatg 3001 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 3061 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 3121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 3181 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag 3241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 3301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 3361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 3421 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc 3481 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 3541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 3601 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 3661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc 3721 ggccgcgact ctagaattcg atatcaagct tatcgataat caacctctgg attacaaaat 3781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 3841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 3901 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 3961 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 4021 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 4081 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 4141 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct 4201 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 4261 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg 4321 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca 4381 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc 4441 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac 4501 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 4561 aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta 4621 cttccctgat tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg 4681 atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga 4741 gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt 4801 attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc 4861 ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 4921 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 4981 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 5041 aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc 5101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca 5161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta 5221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc 5281 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta 5341 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 5401 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 5461 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 5521 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 5581 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 5641 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 5701 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 5761 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc 5821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag 5881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 5941 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 6001 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 6061 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg 6121 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat 6181 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg 6241 gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag 6301 ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg 6361 gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac 6421 accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc 6481 gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg 6541 tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag 6601 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg 6661 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg 6721 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc 6781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 6841 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg 6901 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 6961 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9061 cctgac //