LOCUS Exported 3267 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pMSG360zeo SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3267) AUTHORS Barkan D, Rao V, Sukenick GD, Glickman MS TITLE Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis cis cyclopropanation of oxygenated mycolates. JOURNAL J Bacteriol. 2010 Jul . 192(14):3661-8. PUBMED 20472794 REFERENCE 2 (bases 1 to 3267) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3267 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(97..130) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter 239..269 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" protein_bind 275..294 /label=lac operator (symmetric) /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG). The symmetric lac operator was optimized for tight binding of lac repressor." regulatory 335..344 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 341..715 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" protein_bind complement(767..800) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 1159..1293 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 1467..2055 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1956..1975 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2220..2237 /label=L4440 /note="L4440 vector, forward primer" polyA_signal 2451..2585 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" ORIGIN 1 ctagtgggca gatcttcgaa tgcatcgcgc gcaccgtacg tctcgaggaa ttcctgcagg 61 atatctggat ccacgaagct tgcatggtgg cccggtataa cttcgtataa tgtatgctat 121 acgaagttat ggatcatcct gagcttgcat gcctgcaggt cgtcgaggtc caccaagccc 181 tcggcgacgt tccgccgggc ctcggcgacc gccgcgtcga ggcctccaga ccccaggctt 241 gacactttat gcttccggct cgtataatgt gtggaattgt gagcgctcac aattcgcatg 301 ctgataaatg cttcaataat agcacgtgag gagggccacc atggccaagt tgaccagtgc 361 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct 421 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac 481 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg 541 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg 601 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc 661 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt 721 gcctcgagat aaaattgcag gaattcgatg cctgacgccc cagcttataa cttcgtataa 781 tgtatgctat acgaagttat tcgaactgta ccggatttcg gacaggactc tagaggatcc 841 ccgggtacct gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta 901 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag 961 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt 1021 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca 1081 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct 1141 acgtcttgct ggcgttcgaa cttgtttatt gcagcttata atggttacaa ataaagcaat 1201 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 1261 aaactcatca atgtatctta tcatgtctgg atctgacggg tgcgcatgat cgtgctcctg 1321 tcgttgagga cccggctagg ctcttaagta ccgaattctg caggtttgac agcttatcat 1381 cgcgataagc tcatgaccaa aatcccttaa cgtgagtttt cgttcgactg agcgtcagac 1441 cccgtagaaa atatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1501 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1561 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 1621 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1681 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1741 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1801 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1861 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1921 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1981 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 2041 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 2101 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 2161 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 2221 gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc gcgtttccag actttacgaa 2281 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag 2341 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc 2401 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtca gatccagaca 2461 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct 2521 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac 2581 aagttcgcga attgataatt attatcattt gcgggtcctt tccggcgatc cgccttgtta 2641 cggggcggcg acctcgcggg ttttcgctat ttatgaaaat tttccggttt aaggcgtttc 2701 cgttcttctt cgtcataact taatgttttt atttaaaata ccctctgaaa agaaaggaaa 2761 cgacaggtgc tgaaagcgag ctttttggcc tctgtcgttt cctttctctg tttttgtccg 2821 tggaatgaac aatggaagtc aacaaaaagc aagctagctt atcgattact atcgacggca 2881 ctgctgccag ataacaccac cggggaaaca ttccatcatg atggccgtgc ggacatagga 2941 agccagttca tccatcgctt tcttgtctgc tgccatttgc tttgtgacat ccagcgccgc 3001 acattcagca gcgtttttca gcgcgttttc gatcaacgtt tcaatgttgg tatcaacacc 3061 aggtttaact ttgaacttat cggcactgac ggttaccttg ttctgcgctg gctcatcacg 3121 caggatacca aggctgatgt tgtagatatt ggtcaccggc tgagggtttt cgattgccgc 3181 tgcgtggata gcaccatttg cgatcaggcn gtccttgatg aatgacactc cattgcgaat 3241 aagttcgaag gagacggtgt cacgaaa //