LOCUS Exported 9094 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pAC-GAL4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9094) AUTHORS Potter CJ, Tasic B, Russler EV, Liang L, Luo L. TITLE The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis JOURNAL Volume 141, Issue 3, 536-548, 30 April 2010 PUBMED 20434990 REFERENCE 2 (bases 1 to 9094) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9094 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(10..28) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 96..200 /gene="bla" /label=AmpR promoter CDS 201..1061 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(419..438) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1232..1820 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1721..1740 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1974..1991 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2108..2129 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2187..2209 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2206..2222 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2206..2222 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 4804..7449 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4" /product="GAL4 transcriptional activator" /label=GAL4 /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD EDTPPNPKKE" primer_bind complement(8700..8717) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8700..8716) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8709..8731) /label=M13/pUC Forward /note="In lacZ gene" primer_bind complement(8925..8944) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 9044..9066 /label=pGEX 3' /note="pGEX vectors, reverse primer" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 2221 accatgatta cgaattctat attctaaaaa cacaaatgat acttctaaaa aaaaatcatg 2281 aatggcatca actctgaatc aaatctttgc agatgcacct acttctcatt tccactgtca 2341 catcattttt ccagatctcg ctgcctgtta tgtggcccac aaaccaagac acgttttatg 2401 gccattaaag ctggctgatc gtcgccaaac accaaataca taatgaatat gtacacattc 2461 gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag gagaatgcgg aggagaagga 2521 gaacgagctg atctagtatc tctccacaat ccaatgccaa ctgaccaact ggccatattc 2581 ggagcaattt gaagccaatt tccatcgcct ggcgatcgct ccattcttgg ctatatgttt 2641 ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca agataagatt gtgtcactcg 2701 ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt 2761 ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt ttctacaaaa 2821 acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa gattgtatgt 2881 atataccttt aatgtattct cattttctta tgtatttata atggcaatga tgatactgat 2941 gatattttaa gatgatgcca gaccaaaagg cttgaatttc tgcgtctttt gccgaacgca 3001 gtgcatgtgc aattgttgtt ttttggaata ttcaattttc ggactgtccg ctttgatttc 3061 agtttcttgg cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa 3121 tgcggcaaaa cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg 3181 ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc 3241 atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat 3301 gctgcgctct ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt 3361 tccttttatg cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg 3421 aaaacgctaa aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa 3481 cgatagcaac aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc 3541 cgcgtgcgag caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa 3601 gttttggcga aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata 3661 tttaatattg tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa ataaaacaaa 3721 ttatttgaaa gagaattagg aatcggacgc ttatcgttag ggtaacaaca agaaatgctt 3781 actgagtcac agcctctgga aaactgccgc aagccagaga gagagagaaa aagagggaga 3841 gcagcttaga ccgcatgtgc ttgtgtgtga ggcgtctctc tcttcgtctc tgttgcgcaa 3901 acgcatagac tgcactgaaa aaatcgatta cctatttttt atgaatgaat atttgcacta 3961 ttactattca aaactattaa gatagcaatc acattcaata gccaaatact ataccacctg 4021 agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc atatttagga cggcatcatt 4081 atagaaatgc ttcttgctgt gtacttttct ctcgtctggc agctgtttcg ccgttattgt 4141 taaaaccggc ttaagttagg tgtgttttct acgactagct agtgaatgcc ctactagaag 4201 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg 4261 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa 4321 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa 4381 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat 4441 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt 4501 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt 4561 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga 4621 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac 4681 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc 4741 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatccc 4801 aacatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact taaaaagctc 4861 aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc 4921 tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga agtggaatca 4981 aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga ccttgacatg 5041 attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt atttgtacaa 5101 gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac tgatatgcct 5161 ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag tagtaacaaa 5221 ggtcaaagac agttgactgt atcgattgac tcggcagctc atcatgataa ctccacaatt 5281 ccgttggatt ttatgcccag ggatgctctt catggatttg attggtctga agaggatgac 5341 atgtcggatg gcttgccctt cctgaaaacg gaccccaaca ataatgggtt ctttggcgac 5401 ggttctctct tatgtattct tcgatctatt ggctttaaac cggaaaatta cacgaactct 5461 aacgttaaca ggctcccgac catgattacg gatagataca cgttggcttc tagatccaca 5521 acatcccgtt tacttcaaag ttatctcaat aattttcacc cctactgccc tatcgtgcac 5581 tcaccgacgc taatgatgtt gtataataac cagattgaaa tcgcgtcgaa ggatcaatgg 5641 caaatccttt ttaactgcat attagccatt ggagcctggt gtatagaggg ggaatctact 5701 gatatagatg ttttttacta tcaaaatgct aaatctcatt tgacgagcaa ggtcttcgag 5761 tcaggttcca taattttggt gacagcccta catcttctgt cgcgatatac acagtggagg 5821 cagaaaacaa atactagcta taattttcac agcttttcca taagaatggc catatcattg 5881 ggcttgaata gggacctccc ctcgtccttc agtgatagca gcattctgga acaaagacgc 5941 cgaatttggt ggtctgtcta ctcttgggag atccaattgt ccctgcttta tggtcgatcc 6001 atccagcttt ctcagaatac aatctccttc ccttcttctg tcgacgatgt gcagcgtacc 6061 acaacaggtc ccaccatata tcatggcatc attgaaacag caaggctctt acaagttttc 6121 acaaaaatct atgaactaga caaaacagta actgcagaaa aaagtcctat atgtgcaaaa 6181 aaatgcttga tgatttgtaa tgagattgag gaggtttcga gacaggcacc aaagttttta 6241 caaatggata tttccaccac cgctctaacc aatttgttga aggaacaccc ttggctatcc 6301 tttacaagat tcgaactgaa gtggaaacag ttgtctctta tcatttatgt attaagagat 6361 tttttcacta attttaccca gaaaaagtca caactagaac aggatcaaaa tgatcatcaa 6421 agttatgaag ttaaacgatg ctccatcatg ttaagcgatg cagcacaaag aactgttatg 6481 tctgtaagta gctatatgga caatcataat gtcaccccat attttgcctg gaattgttct 6541 tattacttgt tcaatgcagt cctagtaccc ataaagactc tactctcaaa ctcaaaatcg 6601 aatgctgaga ataacgagac cgcacaatta ttacaacaaa ttaacactgt tctgatgcta 6661 ttaaaaaaac tggccacttt taaaatccag acttgtgaaa aatacattca agtactggaa 6721 gaggtatgtg cgccgtttct gttatcacag tgtgcaatcc cattaccgca tatcagttat 6781 aacaatagta atggtagcgc cattaaaaat attgtcggtt ctgcaactat cgcccaatac 6841 cctactcttc cggaggaaaa tgtcaacaat atcagtgtta aatatgtttc tcctggctca 6901 gtagggcctt cacctgtgcc attgaaatca ggagcaagtt tcagtgatct agtcaagctg 6961 ttatctaacc gtccaccctc tcgtaactct ccagtgacaa taccaagaag cacaccttcg 7021 catcgctcag tcacgccttt tctagggcaa cagcaacagc tgcaatcatt agtgccactg 7081 accccgtctg ctttgtttgg tggcgccaat tttaatcaaa gtgggaatat tgctgatagc 7141 tcattgtcct tcactttcac taacagtagc aacggtccga acctcataac aactcaaaca 7201 aattctcaag cgctttcaca accaattgcc tcctctaacg ttcatgataa cttcatgaat 7261 aatgaaatca cggctagtaa aattgatgat ggtaataatt caaaaccact gtcacctggt 7321 tggacggacc aaactgcgta taacgcgttt ggaatcacta cagggatgtt taataccact 7381 acaatggatg atgtatataa ctatctattc gatgatgaag ataccccacc aaacccaaaa 7441 aaagagtaag cggccgcgtt aaccgatccg tcgaccatga agatcaagat cattgccccg 7501 ccagagcgca agtactctgt ctggatcggt ggctccatcc tggcttcgct gtccaccttc 7561 cagcagatgt ggatctccaa gcaggagtac gacgagtccg gcccctccat tgtgcaccgc 7621 aagtgcttct aagaaggatc gcttgtctgg gcaagaggat caggatcggg atggtcttga 7681 ttctgctgga ggaggaggag gagaagtcga ggaagcagca gcgaaagtgc aagtgcgagt 7741 ggtggaagtt tggagtgcag cacaacaaaa tcaacaacaa caccaactac aagatgaaaa 7801 gagcggaacc acctgcacac catcatcact atcatcatcg ttttgggcgc atgttgtgtg 7861 gttccagcgt attaatataa ttaatttatt ccacatgaga tatgatatga tatactatgt 7921 attttttgtt ttttttttat ttgtaaacct ttaatataac aagaactaca aaaaatgaaa 7981 atgagcgaaa atgcatattc tgccattcca cacacacacc aacaacaccc aacacacgca 8041 cacccacaag cttacacaca cacattcgcg gcatgacaag gacatcaaga taaagaagaa 8101 cttaaagaag atatttccca aagcgcaaaa agaacacaca cacattgcaa aacacaaaca 8161 acacactagc gttttgtaca attcgtcagc aaccttatgt attatttttt aattatgatg 8221 taattataaa caaagtgaaa caaaaatatg aaaacaaaaa ggaaaatcaa atctgtcttc 8281 tctttctccc gctctcctcg ctctctgctg ctaacctcgc cctctcctct ctcatctttt 8341 tgtctgtctc tcttccacat ttttgccggc cggcaaaata ataacccaca cacactcaca 8401 cttggctgca gtttcgcgtg cgatattcac acacattcaa gcatacaagc atacatacat 8461 atgtattttt tttttatttg tacacttttc taattgcatg cgtatcgatt gataagttta 8521 cgcctgaaaa tgttaattaa aatgtgaaaa tgcaactgaa aaactgatga aatgaaacaa 8581 caacaagcga acaatttgct acatgtgtat tgtctaacaa ccgttactgc aacggttgct 8641 tcggaaaagg gtgaagagga agagggcacg aggtcgacct gcaggcatgc aagcttggca 8701 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 8761 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 8821 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt 8881 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat 8941 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 9001 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 9061 cagaggtttt caccgtcatc accgaaacgc gcga //