LOCUS Exported 2162 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]. ACCESSION . VERSION . KEYWORDS R0040 (pTet)_EB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2162) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 2162) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2162 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 5..23 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 30..48 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" misc_feature 70..90 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(96..113) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(267..855) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(347..366) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1026..1886) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1649..1668 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1887..1991) /gene="bla" /label=AmpR promoter primer_bind 2059..2077 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2081..2124 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2127..2148 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 gctttcccta tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta 61 ctagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg 121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 241 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac 301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1141 ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga tttatcagca 1201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 1381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 1441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 1501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 1561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 1621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 1681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 1741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 1801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 1861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 1921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 1981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 2041 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc 2101 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac tagtgggtct 2161 ca //