LOCUS Exported 2259 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]. ACCESSION . VERSION . KEYWORDS R0063 (pLuxR-pR)_FB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2259) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 2259) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2259 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 167..187 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(193..210) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(364..952) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(444..463) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1123..1983) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1746..1765 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1984..2088) /gene="bla" /label=AmpR promoter primer_bind 2156..2174 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2178..2221 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2224..2245 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 cgctacctgt acgatcctac aggtgcttat gttaagtaat tgtattccca gcgatacaat 61 agtgtgacaa aaatccaatt tattagaatc aaatgtcaat ccattaccgt tttaatgata 121 tataacacgc aaaacttgcg acaaacaata ggtaatacta gagacctact agtagcggcc 181 gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 361 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 781 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 1021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 1081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 1141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 1201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 1261 gcgcgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 1321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 1381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 1441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 1501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 1561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 1621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 1681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 1741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 1801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 1861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 1921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 1981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 2041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 2101 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 2161 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt 2221 ctggaattcg cggccgcttc tagagactag tgggtctca //