LOCUS Exported 3023 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]. ACCESSION . VERSION . KEYWORDS C0080 (araC)_CD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3023) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 3023) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3023 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 5..880 /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" CDS 881..913 /codon_start=1 /product="C-terminal peptide that mediates degradation in bacteria through the ClpXP and ClpAP proteases (McGinness et al., 2006)" /label=ssrA tag (LVA) /note="mutant LVA variant that confers accelerated degradation under some conditions (Andersen et al., 1998)" /translation="AANDENYALVA" misc_feature 931..951 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(957..974) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1128..1716) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1208..1227) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1887..2747) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2510..2529 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2748..2852) /gene="bla" /label=AmpR promoter primer_bind 2920..2938 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2942..2985 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2988..3009 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta acgcccatct 61 ggtggcgggt ttaacgccga ttgaggccaa tggttatctc gattttttta tcgaccgacc 121 gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa 181 tcagggacga gaatttgtct gccgaccggg tgatattttg ctgttcccgc caggagagat 241 tcatcactac ggtcgtcatc cggaggctcg cgaatggtat caccagtggg tttactttcg 301 tccgcgcgcc tactggcatg aatggcttaa ctggccgtca atatttgcca atacgggttt 361 ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc aaatcattaa 421 cgccgggcaa ggggaagggc gctattcgga gctgctggcg ataaatctgc ttgagcaatt 481 gttactgcgg cgcatggaag cgattaacga gtcgctccat ccaccgatgg ataatcgggt 541 acgcgaggct tgtcagtaca tcagcgatca cctggcagac agcaattttg atatcgccag 601 cgtcgcacag catgtttgcc tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt 661 agggattagc gtcttaagct ggcgcgagga ccaacgcatc agccaggcga agctgctttt 721 gagcactacc cggatgccta tcgccaccgt cggtcgcaat gttggttttg acgatcaact 781 ctatttctcg cgagtattta aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg 841 ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca gctgcaaacg acgaaaacta 901 cgctttagta gcttaataaa ggtagagacc tactagtagc ggccgctgca ggcttcctcg 961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 1021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 1081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc 1141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 1201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 1261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 1321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 1381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 1441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 1501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 1561 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 1621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 1681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 1741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 1801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 1861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 1921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 1981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgcga cccacgctca 2041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 2101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 2161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 2221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 2281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 2341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 2401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 2461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 2521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 2581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 2641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 2701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 2761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 2821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 2881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 2941 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg 3001 cttctagaga ctagtgggtc tca //