LOCUS Exported 2831 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]. ACCESSION . VERSION . KEYWORDS E0030 (YFP)_CD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2831) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 2831) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2831 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 5..724 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(50..71) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(311..330) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 658..679 /label=EGFP-C /note="EGFP, forward primer" misc_feature 739..759 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(765..782) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(936..1524) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1016..1035) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1695..2555) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2318..2337 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2556..2660) /gene="bla" /label=AmpR promoter primer_bind 2728..2746 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2750..2793 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2796..2817 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aatgatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 61 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 121 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 181 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat 241 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 301 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 361 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 421 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 481 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 541 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 601 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 661 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 721 gtaataaagg tagagaccta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg 781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac 961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 1021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 1081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 1141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 1201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 1261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 1321 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 1381 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 1441 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 1501 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 1561 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1621 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1681 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 1741 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 1801 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat 1861 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 1921 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 1981 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 2041 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 2101 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 2161 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 2221 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 2281 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 2341 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 2401 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 2461 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 2521 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 2581 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 2641 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 2701 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 2761 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact 2821 agtgggtctc a //