LOCUS Exported 2237 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]. ACCESSION . VERSION . KEYWORDS B0015_DE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2237) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 2237) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2237 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 13..84 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 100..127 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 145..165 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(171..188) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(342..930) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(422..441) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1101..1961) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1724..1743 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(1962..2066) /gene="bla" /label=AmpR promoter primer_bind 2134..2152 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 2156..2199 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 2202..2223 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" ORIGIN 1 aggtccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 61 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 121 ttctgcgttt atagcttaga gacctactag tagcggccgc tgcaggcttc ctcgctcact 181 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 241 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 301 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc 361 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 421 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 481 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 541 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 601 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 661 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 721 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 781 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 841 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 901 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 961 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 1021 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1081 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 1141 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 1201 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct 1261 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 1321 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 1381 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1441 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 1501 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 1561 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 1621 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 1681 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 1741 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 1801 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 1861 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 1921 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 1981 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 2041 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 2101 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc 2161 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta 2221 gagactagtg ggtctca //