LOCUS Exported 677 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:G]. ACCESSION . VERSION . KEYWORDS DVA_EG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 677) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 677) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..677 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 9..30 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS complement(25..348) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCRRHKLRP TSL" primer_bind 243..265 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 257..274 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 258..274 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 275..331 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(344..360) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(344..360) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(357..379) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 368..384 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(392..422) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 437..458 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 558..578 /label=BioBrick suffix /note="universal suffix for all parts" primer_bind complement(584..601) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gatttctgga attcgcggcc gcttctagag actagtgggt ctcagcttat gtcttctgca 61 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 121 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 181 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 241 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cccggggatc 301 ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg 361 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 421 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 481 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 541 agacgttgcc agagacctac tagtagcggc cgctgcaggc ttcctcgctc actgactcgc 601 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 661 tatccacaga atcaggg //