LOCUS Exported 841 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION MoClo Destination Vector: (based on pSB1K3) [F:LacZa:G]. ACCESSION . VERSION . KEYWORDS DVK_FG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 841) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 841) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..841 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 22..65 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 68..89 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" CDS complement(84..407) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCRSLAMSS TSL" primer_bind 302..324 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 316..333 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 317..333 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 334..390 /label=MCS /note="pUC18/19 multiple cloning site" primer_bind complement(403..419) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(403..419) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(416..438) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 427..443 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(451..481) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 496..517 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." misc_feature 617..637 /label=BioBrick suffix /note="universal suffix for all parts" terminator 638..695 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(719..736) /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg 61 atttctggaa ttcgcggccg cttctagaga ctagtggaag acatcgctag agacctgcac 121 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat 181 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 241 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 301 tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac ccggggatcc 361 tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt 421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 541 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcgggggt 601 ctcttgccat gtcttctact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt 661 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct 721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 841 a //