LOCUS Exported 820 bp ds-DNA linear SYN 28-MAY-2021 DEFINITION 3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation. ACCESSION . VERSION . KEYWORDS pLenti-CaMKIIa-ChETA-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 820) AUTHORS Gunaydin LA, Yizhar O, Berndt A, Sohal VS, Deisseroth K, Hegemann P TITLE Ultrafast optogenetic control. JOURNAL Nat Neurosci. 2010 Mar . 13(3):387-92. PUBMED 20081849 REFERENCE 2 (bases 1 to 820) AUTHORS . TITLE Direct Submission JOURNAL Exported May 28, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..820 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(33..752) /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(78..99) /label=EGFP-C /note="EGFP, forward primer" primer_bind 427..446 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 686..707 /label=EGFP-N /note="EGFP, reverse primer" regulatory 749..758 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ggttgattat cgataagctt gatatcgaat tcttacttgt acagctcgtc catgccgaga 61 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg 121 tctttgctca gggcggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg 181 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg 241 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag 301 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg 361 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg 421 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct 481 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag 541 cactgcaggc cgtagccgaa ggtggtcacg agggtgggcc agggcacggg cagcttgccg 601 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg 661 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc 721 ccggtgaaca gctcctcgcc cttgctcacc atggtggcgg ccgctggcac ggctccggcc 781 tcggcttcgt cttcgacgag agtctcgacc tcgatctccg //