LOCUS Exported 7065 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pHPyV6-607a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7065) AUTHORS Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB. TITLE Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. JOURNAL Cell Host Microbe. 2010 Jun 25;7(6):509-15. PUBMED 20542254 REFERENCE 2 (bases 1 to 7065) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7065 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 176..198 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 190..207 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 191..207 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 223..322 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" CDS complement(3377..3388) /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" protein_bind complement(5258..5357) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter complement(5954..5972) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter complement(5964..6011) /label=EM7 promoter /note="synthetic bacterial promoter " rep_origin complement(6097..6685) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6177..6196) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind complement(6893..6910) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 7063..84 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat 121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag 181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt 241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca 301 actttgtaca aaaaagcagg ctcaaaagct tcccagagta ataaaaaaag gaggagtgga 361 ggttatggaa acagtgccac tttctgaaga cacaatttat aaagtggagg caattctgct 421 gcctaatttt gcttctggat ccaatactgc agtataccag tctcggggag ccccctacac 481 ctttactgac actttggatg ctggttcatc tctgtgttat accctggctg ttgttaattt 541 gcctgaaatt cctgaggccc tgtgtgatga tactttgttg gtttgggaag ccttcagagt 601 tgaaacagaa ctaatattta ccccacaagt gggaagtgct ggatatataa gagcacaagg 661 aacccctgct ggtgtagaag gttcccaaat gtacttttgg gcctgtggag gcagcccctt 721 ggatgtcatt ggtattaacc cagatccaga aagaatgaat gtagcagcag gcctggaagg 781 gcctagtaaa gagaaccaac catctgttgc aggcattaaa gctaccagaa aacaagttac 841 agctgccaat tttcccattg aaatttggag tgctgatcct accaggaatg agaactgtag 901 atattttggc agaattgttg gtggtagtgt tacccctcca gtggtttcct ttggaaatca 961 gagcaccact ccattggttg atgaaaatgg agtaggtata ctctgtttgt ttggagctat 1021 atatctaaca tcagcagata tgttggggat ggtagggtat gctggtaacc ccactctcag 1081 tgatgcctat agccagcaaa gaagtgtgca ggctgccttt ggaagatttt ttagagttca 1141 cttcagacag aggagagtta aacatccata tactgtggat atgatgttta gacaattcct 1201 gcagcctcag aagccacagg tgcaagggac acaacctaat gctgtccaag aagtagtaat 1261 ggaacaaatg cagccaagca tacttccaac tactctggag ggtgctattg ggtactcacc 1321 cagtacaaag tttattctcc aaaatgggga acttatatac ccttctagta cagtagcagc 1381 aggtgctgca aatttgtttg ggcctccagt agagaaacag acaagcaagg aacctagtaa 1441 gggtgagctg taaacccctc tttctgtgaa agacaaagca tatgctgggt aattgttaaa 1501 caatgaattc tttattgtat caataaatct tacatggaat gcatggagcc aatgccagat 1561 tcttgagtac tgtcatttgt ttcatcattt tcctgtggct gtacaaaaga ttcatctacc 1621 agtacatttg taaaagggtc aatgccagct aaaatatttt ctttcatagt ttggtacatt 1681 ccaaagctaa cccatctttc aaaagtttgc ttccagttaa caacatcttc ctgcagttct 1741 tccataaagt cctccactgg ctcccaccaa attagagctg ccaacaaggt ggtccccttt 1801 tgtaacagcc ttttggacat taatacacaa tctttcaaac atttttgcaa acatggctta 1861 tgcagaaaat aatatcctct ggcaattctg gcctttacag tacatggaat tatatagtca 1921 ttacaggtga tcacagatgg aggaaacagc tgggacacct tgtttagatg cttcctctcc 1981 atatttactg gcacacatcc atctaaataa tccctaaggt tatctaggtt tgtcatccca 2041 cacccttgtg taagattggt attagccata ggtgttccct ttacatcctc aatgacacac 2101 atgaatttgt caatggcaca tcccagttca aagtttatct tatctggagt acaatttaca 2161 ttcaaagaca caccccctac aagatccaat attgcagcag ccaatgttgt tttgccacta 2221 tttatggggc ctttgaacaa cacatttctt tttttaggta tgttttcagt taaaatgctt 2281 agtatttcct ttagtttagt agtaaagcta ggctgcaaac aatctaacca ggccacagcc 2341 cccagtaatt gagtaatttc cacacctcct gcatattgct cagctaggat tgtaaattgc 2401 ttcttaaagg cctgtaatac cagatcctgt ctagttgatt ccaacattaa cacccttctt 2461 tgtgctgcta ctctgtcaca ggcctgctga gcaatagatt tctggttttt agctgctttg 2521 aataattggg cattgtgaaa gtgtacttca tggtgtttat gcactttcac tcccttttta 2581 catttcatac agcttgctgg atcattggca aattctatat aatatcccaa taatgccaga 2641 caatcagtta ggtctgtgtc actagcaaaa gaagctactt gattccaatc acattcctct 2701 tttttggagc cttcctgaaa gtcataactg tgtaaaccct cagccctaga ttcttgtata 2761 acagtaaatt catccccctg caaggccata taacattcag cagattttaa aacagctttg 2821 attaacagaa atgaaatagt acagcatttt gacaaagcat ttagcatagc agacactcta 2881 tgtcttctag gagtaataca gtaaagtata gcccctgtat tatcattgca tttataggcc 2941 ccagtaaaaa ctgcattaaa tgctgactgt agcttatcat acaaggtttc ccatttttca 3001 agggttgtat agctaacaaa gcaggtatag catttattac caagagttgc atggcttaga 3061 taatcatgca ggcaagatgg aaagtcattt ggagcaggat tgggttttct tttcttagga 3121 ggagtgcaag aatacttgct gtcttgagag ttgccttcac cagggtccac attgtcttct 3181 tcttctgagg ctgacaggtg ttcatcacaa tataaatctt catcgtccca gcctctattg 3241 aaatcagccc accattgttc ccagccggga ctgccatatt gtggaggtgg ccttacctgt 3301 aaaataaatt ataagtttca atgcatcact acctggacta tctataaaaa ttgcacttac 3361 caccagatga gcttctctgt aattcccaac caatccaggg ggctttggta gataatgtgg 3421 gtccaccaca taaaagaatc agcttccaca ggtaggccaa accagagaca gtagcacttg 3481 tagcaccagc actttcccca aatagttggc tttcctaaat taatctccac ttgcttttta 3541 tgctcctttt tgagaaggca atgaaggcat ttgcaggaac tgattccttg ttgtacacac 3601 aaacggaaat cccaaacttt ctttttatga aattcagggc ctaagaactc cccaacagtt 3661 aagggaatac cccaatcatc actgacctca gaggaaaagc accaggtggg tgatgaagac 3721 atttggtccc tcagggtggc attcaacttc tcctttaaaa cattcaggcg ttgcattttt 3781 tcaggatccc ctcctttatc aggatgatat tttttacaag ctagtcttat cttctgttgc 3841 ataaggggta aattacccca acaggccatg ctgaggccta taagatccat tagctcccta 3901 acttcttctc tggctaaaag gcgatccatt ttgctgcttg agtagtctct gaatgaaatg 3961 ttaaaatccc agacagcagg tgaaatccct gtgcaggctt acgcaactgg gcagggccat 4021 ttaagctccc ttatctctct aattatagga ggcaataaag gccaccaggc cacctcaatg 4081 tatgagaaaa aaggagagga aatagggcaa ccaaaggtca aaaggaagtt attaggcgag 4141 aaaagcccgc caaatttttt cccagtcata actgaggttg accaccgttg acacaacacc 4201 ctaattagta agttcttcaa tttctgtttt attttgtttt aactcttcag tgacctcacc 4261 gcctgttgac cctggtctta atttctactt aacaggtaag ccatgggaat tctatttagt 4321 cttcctgaaa ttatagcagc tgctgctgtt ggagggggag aggccctgga aattgctggt 4381 ggtctaggag cccttgtatc aggggaagga ctggctacat tagaggctct acagtctgct 4441 gctgctttat ccagtgaagc aactgcagct ctagctgtat ctaatgaggc tgccattgta 4501 ttgtcaacag ttcctgagtt gtcccagacc ctctttggag cacaattact actgtcttct 4561 gtagcaggcg ttggaggtgt tatatacagc aactataacc ctggggaact gtacaaggcg 4621 ccagaaggac caggtggact cggcccaaga gttggaaaca ccaccatggc acttcaactg 4681 tggttgccac aagtttggtc ttggggaggc gcgggtagag gtctccccga ctggcttatc 4741 aacatgcttc gtgaagtgcc gtctcccaca gagatcctca gtgacattgt cagaggaata 4801 tggacttcct actatagagc aggcagggag ataatccaaa gaacagcttc tagggaattg 4861 ggggccttgc tgagtagggt aagagaaact gttatacatg gggctgaaag agctttggaa 4921 gcagcccctg acccagtgca aggcctagta aatctagtaa actatgctgt aaattataat 4981 agacagtggg aaacaagggc cctgttagaa ggaaggcctc tgtttgaggg caatggagta 5041 gtaaactatg atatgcaaaa tcttcctgta aatggaaata atgatcaaag gggggggttt 5101 catgatgaag gtctttgggt aagtttctca gcagagcagg gaaatactgg ccaatactgt 5161 atacctcaat ggctgctttt tgttcttgag gagctggaca aagagattaa ggaagatgcc 5221 ttgtcacaga aaaggaaatg gaccaattca aaagcttacc cagctttctt gtacaaagtt 5281 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa 5341 aataaaatca ttatttgcca tccagctgat aagcaagtaa aacctctaca aatgtggtaa 5401 aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 5461 acagttgcgt acctaaccaa gttcctcttt cagaggttat ttcaggccct gcagggttaa 5521 ttaacaaaaa accccgccgg ggcggggttt tttgttacaa ttcttatcct cagtcctgct 5581 cctctgccac aaagtgcaca cagttgcctg ctgggtctct cagggcaaac tctctccccc 5641 agggctgctc tccaatctct gtcatggcag ggccactggc atccctgaag ttggtggaga 5701 ccacctcact ccactcagca tacagctcat ccagtcctct cacccacacc caagccaggg 5761 tgttgtcagg caccacctgg tcctggactg ctgagatgaa cagggtgaca tcatctctga 5821 ccacacctgc aaagtcatcc tccacaaaat ctctggagaa ccccaacctg tcagtccaga 5881 actcaacagc tccagccaca tccctggctg tgagcactgg gacagcactg gtcaacttgg 5941 ccatgatggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat 6001 taattgtcaa ctacttttga aaaatttaat taagaacatg tgagcaaaag gccagcaaaa 6061 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 6121 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 6181 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 6241 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 6301 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 6361 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 6421 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 6481 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 6541 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 6601 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 6661 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 6721 tcagtggaac gaaaactcac gttaagggat tttggtcatg gctagttaat taacccacgt 6781 ttcaacatgc acatcccagt aatttggaaa cattttgttt ccaaagattc acttaacatt 6841 ggtttagcaa catgaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac 6901 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg 6961 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta 7021 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaa //