LOCUS       Exported                7088 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHPyV7-713a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB.
  TITLE     Merkel cell polyomavirus and two previously unknown polyomaviruses 
            are chronically shed from human skin.
  JOURNAL   Cell Host Microbe. 2010 Jun 25;7(6):509-15.
  PUBMED    20542254
REFERENCE   2  (bases 1 to 7088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     176..198
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     190..207
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     191..207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    223..322
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5281..5380)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(5977..5995)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5987..6034)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(6120..6708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6200..6219)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(6916..6933)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      7086..84
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
       61 aacgctctcc tgagtaggac aaatccgccg ggagaacaac aacaattgca ttcattttat
      121 gtttcaggtt cagggggagg tgtgggaggt tttttaacgc tagcatggat gttttcccag
      181 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt
      241 tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
      301 actttgtaca aaaaagcagg ctcaattgtg gttgccacaa gcgtggcctt ggggaggcgc
      361 agctggaggt gtccccgatt ggctccttga ggtgcttcgt gaagtgccta ctccctcaga
      421 gatcctctat aacattgcca gaggaatctg gacttcctac tacagaacag gaagagagtt
      481 aatacaaaga actgctacta gggaattggc aagcctgttg agtagactaa ggcagaatat
      541 tataaatggg gctaatagag ctatagaaat ggctccagac cctgtacaag gccttctaaa
      601 tctaataaat caggccattg cctacaatag ggactgggaa actagggctc tccttgaggg
      661 gaggccattg tttgaaccag ggggtgttgt catgtatgac acacaaaatc tgccactcag
      721 tggaaataat gaccagaggg gagggtttca tgatgaagga acatgggtta gtttccaagg
      781 ggaggaagga aacactcccc agtatactat acctcaatgg atgctttttg ttcttgagga
      841 gctacaaaaa gaagtgaata aggagaataa acatgccctg tcaaagaaaa ggaaatggac
      901 ccacacagaa acttcccaga gtgataagaa aaggaggcgt tgaagttttg gatacagtac
      961 cactcactga ggaaacacag tacaaagttg aagcagtgtt gctgccaaac tttggaaaag
     1021 cagctacaac tgggaacttt caatctagag gcctacctta tcctatgagt gacactctgg
     1081 ggccgggggc tgctttgtgt tactctgttg ctgttattaa tttgcctgaa attcctgatg
     1141 ccatgtgtga ggacacaatg attgtttggg aggcctatag gctggaaaca gaacttctat
     1201 ttgctccaca aatggctagt tctggttatc aaagggctaa tggcactcta gctggaacag
     1261 agggcagtca actatacttc tgggcctgtg gaggaggccc cttggatgta attggaatta
     1321 accctgaccc tgaaaggcta aaggtcaatg aagccctaga aggtccaggc aatactgatg
     1381 tagctagcct gcaagccctc agaaagcaag taaatgctgc aaatttccca gtagagctct
     1441 gggttgcaga tcccacaaaa aatgataata ccagatactt tggcagagtg gttggagggg
     1501 gagtaacccc tccagttgtg tcctatggga atcagagtac cactcccctt attgatgaaa
     1561 atggagttgg aattttgtgt acttttggca gtgtgtatct taccagtgct gatatggttg
     1621 gaatgacagg ccttccaggc cttcccactc tgagtgctga ctacagtaac cagagaacag
     1681 tacaggctgg ttatggcaga ttttttagag tccactgcag acaaagaaga attaagcacc
     1741 cctacacagt ggatatgatg tttagacagt ttctgcagcc ccagaagcca caggtgcagg
     1801 ggcagcaacc tgctgcagtg caagaagtta caatggagca aatgcagcct gctactatcc
     1861 ctccaactgt tgagggaggc cttggctttg ctcccaccag taagtttctg atccaaaatg
     1921 gagagcttat ttatcccagc agcaatgctg ctgcagcagc tgcaaaaata tctgtagccc
     1981 caaagaaaaa cacagacaac aagaaggaat tgtaaccatt ttattaaact tattccagtg
     2041 gaatacaaag catatgctgg gtaatttcta gaaatgaaaa gttttattgt atcaataaac
     2101 tagttattca tagaaccaat accagactct gtactttctt gggtttccct ggtattttcc
     2161 tctgtgggat cattaatgag cacaccctca aaagggtctt tacctgataa aatattctcc
     2221 ttcatagttt gaaacattcc atatgaaacc cactgttcaa atgtttgttt ccaattaact
     2281 acctcttctt gtatttcttc aacaaagtca gacactggtt cccaccaaat gagagcagct
     2341 agcaatgtac accctttttg caaaagcctt tttttcatta aaatagggtt tgcatccaag
     2401 ctttttttaa ggcctggttt atgcaaaaag tagtagcctt ttgctactct tgccttcact
     2461 gttgttggaa tagcatattc attacaagtt attatacaag ggggaaacag ctgagatgtt
     2521 ttattaatat gcttcctttc caaattcact ggtacacacc catctaaata atccctcata
     2581 ttatcaagat ttgccatccc aacacctgaa ggtaaatctg tgttaggcag gggtgttccc
     2641 tttacatcct caattaccac catgtactta tcaatagcac atcccagttc aaagtttatc
     2701 ttgtcactgc tacaatttac atttaatgcc acccctccta gtaaatccag aatggcagct
     2761 gctaaggttg ttttcccact gttgataggc cctttaaaca ataggtttct tcttttgggg
     2821 gtgttttcta ctaagtagga cagcatctct ttaattttaa tagtgaactg tggcaatagg
     2881 cagtctaacc aggccactgc tcctattaat tgagttattt caacaccccc tccaaactgc
     2941 tctgccagca ctttgaattg tttcttaaat gacataacca tgagatcttt cctagtagat
     3001 tccatcataa gaacccttct ggcagctaaa actctatcag cagcttgctg agcaattccc
     3061 ttttgatttt tagcagtttt aaacagttta gcattggccc agtgcttctc atggtgttgg
     3121 tgggccttca ctgactttaa acacttagga catagtgatg gctctgtagc aaattccata
     3181 taatatccca taataagtaa cacatcagtt aattccatgt ctgctgcaaa atctgctaca
     3241 aaattccagt cacagtcatc ttttttagaa gcttcttgaa aatcataaga gtgaaggcct
     3301 ccttcctttg actctcttaa cacagcaaat ttagaatctt gtaaggcctt ataacattca
     3361 gcatttttta acacagcttt taccataata aaagaaacag tacagtgttt tttacaagca
     3421 tttaaaatag ctgataccct atgtctgctt ccactaataa aaaacaccag ggcaccacta
     3481 ccatctacac agttgtaggc cccaataaac agagcattaa actcaacacc taatttctca
     3541 taaagcaaca tacttttctc ataagttgtg tagcacaaaa agcaggtata gcacttgtta
     3601 cctaaagtag catggcttaa aaactcagac aggcattcag gaaagtcttg gggagctgga
     3661 ttaggctttt tttgcttagg aggggtggca gaatatttac tatcttggct gcatccctcc
     3721 tcaggattca cagaagcaga tgctgcaggt tcttcttcat cagatgatga cagctcttct
     3781 gtacagtaaa gatcttcatc ccatcccttg ttgaagtcct gccaccattg gtcccacgca
     3841 gggcttccat atgggggcgg aggtctcacc tacaaaaaga taattagtaa ggttgctttg
     3901 tttactcccc ttagtcaggc ttttaggcct tggattactt acagtagatt tagctgtccc
     3961 caaaggttaa gtacatccat tggagtttgg aaaataatgt ggctccacca cataaaggat
     4021 tcctcagatc tttctactcc aaaccagtct aaatagcagt caaagcacca gcatttgccc
     4081 cataaaagag gcccccggtg gtctttagca tactctttgg ccttcttctt atgctttctc
     4141 tttaaaatgc aatgaataca acagcaggct cttaaacctt gcactacaca caaattgtag
     4201 ttccacacct tttttctgtt aaactctggc cccaaaaact cccctacagt caattctata
     4261 tcccaaaaac tgacctcatc agagctgtaa tgccacatgg ggcttccaga cctttgatct
     4321 ctcaaagtag cttgcagctt gtcttttaag acattcagtc tttgcatttt ctggggatct
     4381 ccacctttat ctggatgata ctttttgctg gctaatctaa ccttatgctg tatgaggggc
     4441 aaattccccc aacaggccat gttcaggcct attaattcca taagctcctt gacttcatct
     4501 ctacctaaaa gcttgtccat tttccctttc tctctgtaat ttctgaagag aaagtggtgg
     4561 caggtgcagc tgacaccctt taaatagtct tctctctgca atagaagggt ggaggcaaga
     4621 ggccaccaag ccacctacat taggaaaaaa aaggtggagg aggaaatgtc tgggttactg
     4681 ggtcagacag gatatgatta gcataatatt agcccgccaa aagctgtggt ttttatgcag
     4741 atgaggtgtg acctttggtg gaggacctat ctctctatct gtaagtaaga atttttcact
     4801 ttttaacaaa ttttaacctc ttagtaactg tagtgttctc tctcctcaga aagtccttga
     4861 tattgttggc catttgggtg ctgtgtcaag gacttaggta agagaatggg tattttgtta
     4921 agtattcctg agatcattgc agctactgct attggtggag gggaggcctt ggaaattgct
     4981 ggaagtgtag gagctttggt gtctggagag ggattggcca ccttggaagc ccttcaaagt
     5041 gctgctgctc tatctactga ggccacagct gctctggcag tgtccaatga agctgctata
     5101 gtattatcta ctattcctga attatcccag accttgtttg gagtccaaac cttactatct
     5161 tctgtggcag gtgttggtgg tgtagtatac aacctaaatc ctggggaact gtaccaggcg
     5221 cccgagggac caggtggact cggcccaaga attggaagca caaccatggc acttcaattg
     5281 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     5341 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gataagcaag
     5401 taaaacctct acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa tagcgaagag
     5461 gcccgcaccg atcgcccttc ccaacagttg cgtacctaac caagttcctc tttcagaggt
     5521 tatttcaggc cctgcagggt taattaacaa aaaaccccgc cggggcgggg ttttttgtta
     5581 caattcttat cctcagtcct gctcctctgc cacaaagtgc acacagttgc ctgctgggtc
     5641 tctcagggca aactctctcc cccagggctg ctctccaatc tctgtcatgg cagggccact
     5701 ggcatccctg aagttggtgg agaccacctc actccactca gcatacagct catccagtcc
     5761 tctcacccac acccaagcca gggtgttgtc aggcaccacc tggtcctgga ctgctgagat
     5821 gaacagggtg acatcatctc tgaccacacc tgcaaagtca tcctccacaa aatctctgga
     5881 gaaccccaac ctgtcagtcc agaactcaac agctccagcc acatccctgg ctgtgagcac
     5941 tgggacagca ctggtcaact tggccatgat ggccctccta tagtgagtcg tattatacta
     6001 tgccgatata ctatgccgat gattaattgt caactacttt tgaaaaattt aattaagaac
     6061 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6541 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6781 atggctagtt aattaaccca cgtttcaaca tgcacatccc agtaatttgg aaacattttg
     6841 tttccaaaga ttcacttaac attggtttag caacatgaag cttggctgtt ttggcggatg
     6901 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     6961 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     7021 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     7081 ggcatcaa
//
