LOCUS       Exported                 968 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  Intron targeting-mediated EGFP knockin at the zebrafish gfap locus.
ACCESSION   .
VERSION     .
KEYWORDS    gfap-EGFP donor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
  TITLE     Intron targeting-mediated and endogenous gene integrity-maintaining 
            knockin in zebrafish using the CRISPR/Cas9 system.
  JOURNAL   Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
  PUBMED    25849248
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             265..783
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(310..331)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(571..590)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     918..939
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctcggtacct aagccgggaa tcgaactcgg atcaccataa gaaccatggt gctatgtgtt
       61 gcgcacttaa ccactaggct ataggcgccg accgattaag ttaacttcaa ttaaagtgga
      121 ttaaacataa aacaattaag ttgtccccaa aaacatctca agaattgtgt tgattctgct
      181 catttttcat tttacacatt ctctctttcg cagatcatta aagagtccac tacggagagg
      241 aaggatctgc caggatcctc ttctatggtg agcaagggcg aggagctgtt caccggggtg
      301 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      361 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      421 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      481 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      541 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      601 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      661 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      721 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      781 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      841 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      901 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      961 ggcatgga
//
