LOCUS       Exported                2679 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Leader Of Cox8 Repeated (LOC8R) is a synthetic mitochondrial 
            targeting sequence compatible with any N1 vector. Internal 
            restriction sites enable modular subcloning of 1x, 2x, 3x or 4x 
            presequences..
ACCESSION   .
VERSION     .
KEYWORDS    N1-LOC8R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2679)
  TITLE     Mitochondrial LOC8R Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             343..354
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             382..456
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             478..552
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             574..648
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             670..744
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     complement(885..902)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(995..1583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1075..1094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1757..2551)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2552..2656)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca
      361 aggccgcata agcttgccac catgagcgtg ctgacccccc tgctgctgag aggcctgaca
      421 ggctctgcta gaaggctgcc tgtgcctcga gccaagatcc acagcctggg ggatccaatg
      481 tccgtgctga cacctctgct gctgcgggga ctgaccggaa gtgctcggag actgccagtg
      541 cccagggcca agattcactc tctgggggat cccatgagtg tgctgactcc actgctgctg
      601 cgcggcctga ctggatcagc acgccgcctg ccagtgcctc gagctaagat tcattccctg
      661 ggcgacccta tgtctgtgct gacccctctg ctgctgaggg ggctgactgg atctgcaagg
      721 cggctgcctg tgccaagggc caaaatccat agtctgggcg acccaccggt ctgggcctca
      781 tgggccttcc tttcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      841 catggtcata gctgtttcct tgcgtattgg gcgctctccg cttcctcgct cactgactcg
      901 ctgcgctcgg tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg
      961 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1021 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1081 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1141 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1201 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1261 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1321 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1381 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1441 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1501 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1561 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1621 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1681 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1741 cttggtctga cagttattag aaaaattcat ccagcagacg ataaaacgca atacgctggc
     1801 tatccggtgc cgcaatgcca tacagcacca gaaaacgatc cgcccattcg ccgcccagtt
     1861 cttccgcaat atcacgggtg gccagcgcaa tatcctgata acgatccgcc acgcccagac
     1921 ggccgcaatc aataaagccg ctaaaacggc cattttccac cataatgttc ggcaggcacg
     1981 catcaccatg ggtcaccacc agatcttcgc catccggcat gctcgctttc agacgcgcaa
     2041 acagctctgc cggtgccagg ccctgatgtt cttcatccag atcatcctga tccaccaggc
     2101 ccgcttccat acgggtacgc gcacgttcaa tacgatgttt cgcctgatga tcaaacggac
     2161 aggtcgccgg gtccagggta tgcagacgac gcatggcatc cgccataatg ctcacttttt
     2221 ctgccggcgc cagatggcta gacagcagat cctgacccgg cacttcgccc agcagcagcc
     2281 aatcacggcc cgcttcggtc accacatcca gcaccgccgc acacggaaca ccggtggtgg
     2341 ccagccagct cagacgcgcc gcttcatcct gcagctcgtt cagcgcaccg ctcagatcgg
     2401 ttttcacaaa cagcaccgga cgaccctgcg cgctcagacg aaacaccgcc gcatcagagc
     2461 agccaatggt ctgctgcgcc caatcatagc caaacagacg ttccacccac gctgccgggc
     2521 tacccgcatg caggccatcc tgttcaatca tactcttcct ttttcaatat tattgaagca
     2581 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2641 aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
