LOCUS Exported 815 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]. ACCESSION . VERSION . KEYWORDS pJ02B2Gm_AE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 815) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 815) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..815 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 22..79 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." misc_feature 80..100 /label=BioBrick suffix /note="universal suffix for all parts" terminator 119..146 /label=T7Te terminator /note="phage T7 early transcription terminator" terminator 162..233 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind complement(296..316) /label=GFP-F /note="GFP, forward primer. Does NOT anneal to EGFP" ORIGIN 1 agcctgcata acgcgaagta atcttttcgg ttttaaagaa aaagggcagg gtggtgacac 61 cttgcccttt tttgccggac tgcagcggcc gctactagta gaagacataa gctataaacg 121 cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt caccgacaaa 181 caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg 241 gacctacctt tattatttgt atagttcatc catgccatgt gtaatcccag cagctgttac 301 aaactcaaga aggaccatgt gatctctctt ttcgttggga tctttcgaaa gggcagattg 361 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg 421 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt 481 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt 541 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc 601 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc 661 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa 721 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa 781 agtagtgaca agtgttggcc atggaacagg tagtt //