LOCUS       Exported                1022 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    AP56-7
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    24..44
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        45..644
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
ORIGIN
        1 tgaccatgat tacgccaagc tatcaacttt gtatagaaaa gttggcacct ttggtctttt
       61 attgtcaact tccattggtt cttccattgt ttctgttaaa ttaatgaatt tttcataaaa
      121 taaagacatt atacaatata aaaatgaaga atttattgaa aataaactgc cagagagaaa
      181 aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt acggtacatt ttcgtgctag
      241 gagttagatg tgcaggcagc aacgagaggg ggagagattt ttttgggcct tgtgaaatta
      301 acgtgagttt tctggtcatc tgactaatca tgttggtttt ttgttggttt attttgtttt
      361 tatctttgtt tttatccaga ttaggaaatt taaattttat gaatttataa tgaggtcaaa
      421 cattcagtcc cagcgttttt cctgttctca ctgtttagtc gaatttttat tttaggcttt
      481 caacaaatgt tctaactgtc ttatttgtga cctcactttt tatatttttt taatttttaa
      541 aaatattaga agtttctagg ataatttttt cgacttttat tctctctacc gtccgcactc
      601 ttcttacttt taaattaaat tgtttttttt tcagttggga aacactttgc tctagaaaaa
      661 atggacaaaa aatacagcat cggccttgac atcggaacga actccgtcgg atgggccgtc
      721 atcaccgacg agtacaaggt cccatccaag aagttcaagg tcctcggaaa caccgaccgt
      781 cactccatca agaagaacct catcggagcc ctcctcttcg actccggaga gaccgccgag
      841 gccacccgtc tcaagcgtac cgcccgtcgt cgttacaccc gtcgtaagaa ccgtatctgc
      901 tacctccaag agatcttctc caacgagatg gccaaggtcg acgactcctt cttccaccgt
      961 ctcgaggagt ccttcctcgt cgaggaggac aagaagcacg agcgtcaccc aatcttcgga
     1021 aa
//
