LOCUS Exported 5977 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION G protein beta-1 tagged with HaloTag on amino terminus. ACCESSION . VERSION . KEYWORDS HaloTag-G protein beta-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5977) TITLE Berlot Lab HaloTag Fusions JOURNAL Unpublished REFERENCE 2 (bases 1 to 5977) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5977 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 138..517 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 518..721 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 672..692 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 718..742 /label=LNCX /note="Human CMV promoter, forward primer" intron 857..989 /label=chimeric intron /note="chimera between introns from human beta-globin and immunoglobulin heavy chain genes" primer_bind 972..991 /label=pMT2-F /note="Synthetic intron, forward primer" promoter 1034..1052 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 1075..1084 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(3051..3068) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 3086..3207 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3097..3116) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3151..3170 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 3393..3848 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3480..3499) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3690..3711 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3915..3934) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4034..4056 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(4094..4112) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4180..4284 /gene="bla" /label=AmpR promoter CDS 4285..5145 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4503..4522) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5316..5904 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5805..5824 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc 1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc 1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac 1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat 1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat 1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag 1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat 1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac 1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga 1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt 1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga 1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc 1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc 1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc 1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg 1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggctc cggtgggggc 1981 gggagcatga gtgagcttga ccagttacgg caggaggccg agcaacttaa gaaccagatt 2041 cgagacgcca ggaaagcatg tgcagatgca actctctctc agatcacaaa caacatcgac 2101 ccagtgggaa gaatccaaat gcgcacgagg aggacactgc gggggcacct ggccaagatt 2161 tacgccatgc actggggcac agactccagg cttctcgtca gtgcctcgca ggatggtaaa 2221 cttatcatct gggacagcta caccaccaac aaggtccacg ccatccctct gcgctcctcc 2281 tgggtcatga cctgtgcata tgccccttct gggaactatg tggcctgcgg tggcctggat 2341 aacatttgct ccatttacaa tctgaaaact cgtgagggga acgtgcgcgt gagtcgtgag 2401 ctggcaggac acacaggtta cctgtcctgc tgccgattcc tggatgacaa tcagatcgtc 2461 accagctctg gagacaccac gtgtgccctg tgggacatcg agaccggcca gcagacgacc 2521 acgtttaccg gacacactgg agatgtcatg agcctttctc ttgctcctga caccagactg 2581 ttcgtctctg gtgcttgtga tgcttcagcc aaactctggg atgtgcgaga aggcatgtgt 2641 cggcagacct tcactggcca cgagtctgac atcaatgcca tttgcttctt tccaaatggc 2701 aatgcatttg ccactggctc agacgacgcc acctgcaggc tgtttgacct tcgtgctgac 2761 caggagctca tgacttactc ccatgacaac atcatctgcg ggatcacctc tgtctccttc 2821 tccaagagcg ggcgcctcct ccttgctggg tacgacgact tcaactgcaa cgtctgggat 2881 gcactcaaag ccgaccgggc aggtgtcttg gctgggcatg acaaccgcgt cagctgcctg 2941 ggcgtgactg acgatggcat ggctgtggcg acagggtcct gggatagctt cctcaagatt 3001 tggaactaag gatccgccgg ctaatagtta attaagtagg cggccgcttc cctttagtga 3061 gggttaatgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact 3121 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta 3181 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag 3241 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa 3301 atccgataag gatcgatccg ggctggcgta atagcgaaga ggcccgcacc gatcgccctt 3361 cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc 3421 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 3481 tcctttcgct ttcttccctt cctttctcgc cacgttcgcg ggctttcccc gtcaagctct 3541 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 3601 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 3661 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 3721 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 3781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 3841 tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 3901 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 3961 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 4021 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 4081 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 4141 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 4201 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 4261 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 4321 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 4381 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 4441 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 4501 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 4561 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 4621 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 4681 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 4741 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 4801 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 4861 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 4921 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 4981 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 5041 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 5101 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 5161 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 5221 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 5281 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 5341 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 5401 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 5461 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 5521 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 5581 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 5641 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 5701 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 5761 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 5821 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 5881 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 5941 ttttgctggc cttttgctca catggctcga cagatct //