LOCUS       Exported                 688 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  synchronize trafficking of ManII from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    pCDH_Str-KDEL_ManII-SBP-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             527..640
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
       61 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
      121 gataagcttg ccacaacccg ggaggcgcgc catgaagtta agtcgccagt tcaccgtgtt
      181 tggcagcgcg atcttctgcg tcgtaatctt ctcactctac ctgatgctgg acaggggtca
      241 cttggactac cctcggggcc cgcgccagga gggctccttt ccgcagggcc agctttcaat
      301 attgcaagaa aagattgacc atttggagcg tttgctcgct gagaacaacg agattatctc
      361 aaatatcaga gactcagtca tcaacctgag cgagtctgtg gaggacggcc cgcgggggtc
      421 accaggcaac gccagccaag gctccatcca cctccactcg ccacagttgg ccctgcaggc
      481 tgaccccaga gactgtttgg atcccaccgg tcgccaccgg aattccatgg acgagaagac
      541 cactggttgg cgaggtggac acgttgttga aggactggct ggggaacttg aacaacttcg
      601 tgcacgactg gagcatcacc cacaaggtca acgtgaacca cctgcaggta tggtgagcaa
      661 gggcgaggag gataacatgg ccatcatc
//
