LOCUS Exported 802 bp ds-DNA linear SYN 13-JUN-2021 DEFINITION Vector for PhiC31o mRNA synthesis. ACCESSION . VERSION . KEYWORDS pBBK SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 802) AUTHORS Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB TITLE One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). JOURNAL BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. PUBMED 25887549 REFERENCE 2 (bases 1 to 802) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..802 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..55 /label=T7 /note="T7 promoter, forward primer" promoter 36..54 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 122..131 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc 61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt 121 cgccaccatg gatacctacg ccggagccta cgacagacag agccgggaga gagagaacag 181 cagcgccgcc agccccgcca cccagagaag cgccaacgag gataaggccg ccgatctgca 241 gagagaggtg gagagggacg gcggcagatt cagatttgtg ggccacttca gcgaggcccc 301 tggcaccagc gccttcggca ccgccgagag acccgagttc gagagaatcc tgaacgagtg 361 tagggccggc aggctgaaca tgatcatcgt gtacgacgtg tcccggttca gcaggctgaa 421 ggtgatggac gccatcccta tcgtgtccga gctgctggcc ctgggcgtga ccatcgtgtc 481 cacccaggaa ggcgtcttta gacagggcaa cgtgatggac ctgatccacc tgatcatgag 541 gctggacgcc agccacaagg agagcagcct gaagagcgcc aagatcctgg acaccaagaa 601 cctgcagagg gagctgggcg gctatgtggg cggcaaggcc ccctacggct tcgagctggt 661 gtccgagacc aaggagatca cccggaacgg caggatggtg aacgtggtga tcaacaagct 721 ggcccacagc accacccccc tgaccggccc cttcgagttt gagcccgacg tgatcaggtg 781 gtggtggcgg gagatcaaga cc //