LOCUS       Exported                9432 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAL119-hCTLA4-Ig
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Guillot C, Menoret S, Guillonneau C, Braudeau C, Castro MG, 
            Lowenstein P, Anegon I
  TITLE     Active suppression of allogeneic proliferative responses by 
            dendritic cells after induction of long-term allograft survival by 
            CTLA4Ig.
  JOURNAL   Blood. 2003 Apr 15. 101(8):3325-33.
  PUBMED    12515725
REFERENCE   2  (bases 1 to 9432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     promoter        577..780
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     731..751
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     777..801
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     gap             1529..2528
                     /estimated_length=1000
     CDS             6984..7175
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7169..7191)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7277..7417
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7332..7351
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7432..7449)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7603..8191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7683..7702)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8362..9222)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8985..9004
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9223..9327)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9395..9413
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtanacng gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccnag taanatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataattnt gtgttactca tagcgcgtaa tatttgtcta gggccgcggg
      361 gactttgacc gtttacgtgg agactcgccc aggtgttttt ctcaggtgtt ttccgcgttc
      421 cgggtcaaag ttggcgtttt attattatag tcagctctag actcnagaag cttgcatgcc
      481 tgcaggtcaa ttccctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      541 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      601 aatgggcgtg gatagcggtt tgactcacgg ggatttncaa gtctccaccc cattgacgtc
      661 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      721 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      781 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      841 agacaccggg accgatccag cctggggatc cctcgaggag ctcagcacat ttgcccccca
      901 gccatggctt gtcttggact ccggaggtac aaagctcaac tgcagctgcc ttctaggact
      961 tggccttttg tagccctgct cactcttctt ttcatcccag tcttctctga agccatacag
     1021 gtgacccaac cttcagtggt gttggctagc agccatggtg tcgccagctt tccatgtgaa
     1081 tattcaccat cacacaacac tgatgaggtc cgggtgactg tgctgcggca gacaaatgac
     1141 caaatgactg aggtctgtgc cacgacattc acagagaaga atacagtggg cttcctagat
     1201 taccccttct gcagtggtac ctttaatgaa agcagagtga acctcaccat ccaaggactg
     1261 agagctgttg acacgggact gtacctctgc aaggtggaac tcatgtaccc accgccatac
     1321 tttgtgggca tgggcaacgg gacgcanatt tatgtcattg atccagaacc atgcccggat
     1381 tctggtaagt ataagcttgc atgcctgcag gtcgatcctg cctggacgca tcccggctat
     1441 gcagccccag tccagggcag caaggcaggc cccgtctgcc tcttcacccg gaggcctctg
     1501 cccgccccac tcatgctcan ggagagggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag
     2581 ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc
     2641 gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg
     2701 ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg
     2761 cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg
     2821 cagaagagcc tctccctgtc tccgggtaaa tgagtgcgac ggccggcaag cccccgctcc
     2881 ccgggctctc gcggtcgcac gaggatgctt ggcacgtacc ccctgtacat acttcccggg
     2941 cgcccagcat ggaaataaag ggatcccacg tcactattgt atactctata ttatactcta
     3001 tgttatactc tgtaatccta ctcaataaac gtgtcacgcc tgtgaaaccg tactaagtct
     3061 cccgtgtctt cttatcacca tcaggtgaca tcctcgccca ggctgtcaat catgccggta
     3121 tcgattccag tagcaccggc cccacgctga caacccactc ttgcagcgtt agcagcgccc
     3181 ctcttaacaa gccgaccccc accagcgtcg cggttactaa cactcctctc cccgacctgc
     3241 agcccaagct tctcgagtct agagtggaag gtgctgaggt acgatgagac ccgcaccagg
     3301 tgcagaccct gcgagtgtgg cggtaaacat attaggaacc agcctgtgat gctggatgtg
     3361 accgaggagc tgaggcccga tcacttggtg ctggcctgca cccgcgctga gtttggctct
     3421 agcgatgaag atacagattg aggtactgaa atgtgtgggc gtggcttaag ggtgggaaag
     3481 aatatataag gtgggggtct tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc
     3541 atgagcacca actcgtttga tggaagcatt gtgagctcat atttgacaac gcgcatgccc
     3601 ccatgggccg gggtgcgtca gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg
     3661 cccgcaaact ctactacctt gacctacgag accgtgtctg gaacgccgtt ggagactgca
     3721 gcctccgccg ccgcttcagc cgctgcagcc accgcccgcg ggattgtgac tgactttgct
     3781 ttcctgagcc cgcttgcaag cagtgcagct tcccgttcat ccgcccgcga tgacaagttg
     3841 acggctcttt tggcacaatt ggattctttg acccgggaac ttaatgtcgt ttctcagcag
     3901 ctgttggatc tgcgccagca ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt
     3961 taaaacataa ataaaaaacc agactctgtt tggatttgga tcaagcaagt gtcttgctgt
     4021 ctttatttag gggttttgcg cgcgcggtag gcccgggacc agcggtctcg gtcgttgagg
     4081 gtcctgtgta ttttttccag gacgtggtaa aggtgactct ggatgttcag atacatgggc
     4141 ataagcccgt ctctggggtg gaggtagcac cactgcagag cttcatgctg cggggtggtg
     4201 ttgtagatga tccagtcgta gcaggagcgc tgggcgtggt gcctaaaaat gtctttcagt
     4261 agcaagctga ttgccagggg caggcccttg gtgtaagtgt ttacaaagcg gttaagctgg
     4321 gatgggtgca tacgtgggga tatgagatgc atcttggact gtatttttag gttggctatg
     4381 ttcccagcca tatccctccg gggattcatg ttgtgcagaa ccaccagcac agtgtatccg
     4441 gtgcacttgg gaaatttgtc atgtagctta gaaggaaatg cgtggaagaa cttggagacg
     4501 cccttgtgac ctccaagatt ttccatgcat tcgtccataa tgatggcaat gggcccacgg
     4561 gcggcggcct gggcgaagat atttctggga tcactaacgt catagttgtg ttccaggatg
     4621 agatcgtcat aggccatttt tacaaagcgc gggcggaggg tgccagactg cggtataatg
     4681 gttccatccg gcccaggggc gtagttaccc tcacagattt gcatttccca cgctttgagt
     4741 tcagatgggg ggatcatgtc tacctgcggg gcgatgaaga aaacggtttc cggggtaggg
     4801 gagatcagct gggaagaaag caggttcctg agcagctgcg acttaccgca gccggtgggc
     4861 ccgtaaatca cacctattac cggctgcaac tggtagttaa gagagctgca gctgccgtca
     4921 tccctgagca ggggggccac ttcgttaagc atgtccctga ctcgcatgtt ttccctgacc
     4981 aaatccgcca gaaggcgctc gccgcccagc gatagcagtt cttgcaagga agcaaagttt
     5041 ttcaacggtt tgagaccgtc cgccgtaggc atgcttttga gcgtttgacc aagcagttcc
     5101 aggcggtccc acagctcggt cacctgctct acggcatctc gatccagcat atctcctcgt
     5161 ttcgcgggtt ggggcggctt tcgctgtacg gcagtagtcg gtgctcgtcc agacgggcca
     5221 gggtcatgtc tttccacggg cgcagggtcc tcgtcagcgt agtctgggtc acggtgaagg
     5281 ggtgcgctcc gggctgcgcg ctggccaggg tgcgcttgag gctggtcctg ctggtgctga
     5341 agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg gtgtcatagt
     5401 ccagcccctc cgcggcgtgg cccttggcgc gcagcttgcc cttggaggag gcgccgcacg
     5461 aggggcagtg cagacttttg agggcgtaga gcttgggcgc gagaaatacc gattccgggg
     5521 agtaggcatc cgcgccgcag gccccgcaga cggtctcgca ttccacgagc caggtgagct
     5581 ctggccgttc ggggtcaaaa accaggtttc ccccatgctt tttgatgcgt ttcttacctc
     5641 tggtttccat gagccggtgt ccacgctcgg tgacgaaaag gctgtccgtg tccccgtata
     5701 cagacttgag aggcctgtcc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     5761 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     5821 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     5881 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     5941 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     6001 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     6061 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     6121 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     6181 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
     6241 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     6301 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     6361 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     6421 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     6481 gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     6541 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     6601 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     6661 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     6721 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     6781 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     6841 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     6901 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     6961 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     7021 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     7081 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     7141 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     7201 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     7261 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     7321 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     7381 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     7441 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7501 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8041 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8281 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8341 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8401 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8461 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8521 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8581 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8641 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     8701 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8761 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8821 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8881 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8941 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     9001 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9061 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9121 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9181 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9241 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9301 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9361 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9421 tcttcaagaa tt
//
