LOCUS Exported 787 bp ds-DNA linear SYN 03-JUN-2021 DEFINITION Vector for iCre mRNA synthesis. ACCESSION . VERSION . KEYWORDS pBBI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 787) AUTHORS Ohtsuka M, Miura H, Hayashi H, Nakaoka H, Kimura M, Sato M, Gurumurthy CB, Inoko H TITLE Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific recombination. JOURNAL Transgenic Res. 2013 Aug;22(4):873-5. doi: 10.1007/s11248-013-9703-x. Epub 2013 Mar 26. PUBMED 23529203 REFERENCE 2 (bases 1 to 787) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..787 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..55 /label=T7 /note="T7 promoter, forward primer" promoter 36..54 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 122..131 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 134..154 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc 61 caagctggct agttaagctt ggtaccgagc tcggatccac tagtccagtg tggtggaatt 121 cgccaccatg gtgcccaaga agaagaggaa agtctccaac ctgctgactg tgcaccaaaa 181 cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc aggaagaacc tgatggacat 241 gttcagggac aggcaggcct tctctgaaca cacctggaag atgctcctgt ctgtgtgcag 301 atcctgggct gcctggtgca agctgaacaa caggaaatgg ttccctgctg aacctgagga 361 tgtgagggac tacctcctgt acctgcaagc cagaggcctg gctgtgaaga ccatccaaca 421 gcacctgggc cagctcaaca tgctgcacag gagatctggc ctgcctcgcc cttctgactc 481 caatgctgtg tccctggtga tgaggagaat cagaaaggag aatgtggatg ctggggagag 541 agccaagcag gccctggcct ttgaacgcac tgactttgac caagtcagat ccctgatgga 601 gaactctgac agatgccagg acatcaggaa cctggccttc ctgggcattg cctacaacac 661 cctgctgcgc attgccgaaa ttgccagaat cagagtgaag gacatctccc gcaccgatgg 721 tgggagaatg ctgatccaca ttggcaggac caagaccctg gtgtccacag ctggtgtgga 781 gaaggcc //