LOCUS       Exported                 781 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synchronize trafficking of TNF from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    Str-KDEL_TNF-SBP-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(120..233)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
       61 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat acctgcaggt
      121 ggttcacgtt gaccttgtgg gtgatgctcc agtcgtgcac gaagttgttc aagttcccca
      181 gccagtcctt caacaacgtg tccacctcgc caaccagtgg tcttctcgtc catggaattc
      241 aaaggcctag gtgcgccatg agctcggccc tcgaggccca gagcaatgat gccaaagtag
      301 acctggccag attcggcaaa gtcgaggtag tcgggtcgat tgatctcagc actcagtcta
      361 tcgcccttct ccagctggaa cacgcctccg aggtagatgg gttcgtacca aggtttagcc
      421 tcagcgccct caggtgtttc ccgctgacat ggggatttga tggcagagag cagattgact
      481 ttagtctggt aagacacggc gatccgtgaa attgtgtgtg tgagcagcac atgtgtagaa
      541 ggacatccct ggcccttaaa cagcacctgg gagtagatga ggtacagtcc ctcactaggc
      601 acgacgagct ggttatcccg gagttccacg ccattggcga gcagagcgtt tgccctccta
      661 ttgagccact ggagctggcc ctcggcctgt gggttggcga cgacatgggc gacaggtttg
      721 tcggatggag tccgagatga agatcggaca gcctgtgcca gtggtgaaat gagtgacaga
      781 t
//
