LOCUS       Exported                 971 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  Bacterial expression plasmid for S. thermophilus1 Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    MSP1673
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, 
            Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas9 nucleases with altered PAM specificities.
  JOURNAL   Nature. 2015 Jun 22. doi: 10.1038/nature14592.
  PUBMED    26098369
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(167..185)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(254..274)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gggtgttgat ttcggcatga agccttatct ttgtagcttc tgcaagattt aagtaactgt
       61 gtaagtttcg ttgtacagtt acttaaatct tgagagtaca aaaacgagtg caggtactag
      121 gtgttggtga atctgttccg aacaacgcta tggcatgacc tgcagtccta tagtgagtcg
      181 tattaatttc gattatgcgg ccgtgtacaa tacgattact ttctgttcga cttaagcatt
      241 atgcggccgc tcacaccttc ctcttcttct tggggtcagc cctgctgaag tccaacttgg
      301 gcttgtcgcc ctcgttcttg atgatgtgct ggttgcccag cacgtcggtg cgcaccttgt
      361 agatgctgat gttgctcttg cccaggccct tcttgcactg gccgctgttg gccacgttgc
      421 ccagcacctt gatcagggcc tcgccgccct cgaacttctg cttgtcgtag ggcttcagct
      481 ccacgtagtg cttctgcttg ggcatggtgc ggctcaggaa gcggaacagc tgttgctcct
      541 tggtctcggt gtccttcacc agcagaaggt cgttcttgta cagggtgaac ttgaactcgc
      601 tgtcgctgtc cacgccctcc ttcttcttga tgtcgttgta cttctcctgg ctgatcttgt
      661 aggtgcctgt gcctttctca aactgcagat cggcgtactt cagccccagg atctcgtact
      721 tgccggtggt cttgttgaag tacacgtcgg cgcgccaggg gctcacgctc tgcagcacca
      781 ccttgttgtt gctgtccttg ggggtgatgt cgatgtggtt gcccagcttg ctgtcgtagt
      841 acttcaggct cttgatctcg gggccgttgc ccttcttgct gtacttgcgg atgtagccgt
      901 gctcctcctt gtacttcagg aaggggttgc agggcacctc cttgcctttc tcgttgatct
      961 gcttgttggg g
//
