LOCUS Exported 890 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION Bacterial expression plasmid for S. thermophilus1 Cas9. ACCESSION . VERSION . KEYWORDS MSP1673 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 890) AUTHORS Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JJ, Aryee MJ, Joung JK TITLE Engineered CRISPR-Cas9 nucleases with altered PAM specificities. JOURNAL Nature. 2015 Jun 22. doi: 10.1038/nature14592. PUBMED 26098369 REFERENCE 2 (bases 1 to 890) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..890 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 118..137 /label=T7 /note="T7 promoter, forward primer" promoter 118..136 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 137..161 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca 61 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa 121 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctgtagaa ataattttgt 181 ttaactttaa taaggagata taccatgggc agcgacctgg tgctgggcct ggacatcggc 241 atcggcagcg tgggcgtggg catcctgaac aaggtgaccg gcgagatcat ccacaagaac 301 agtcgcatct tccctgctgc tcaggctgag aacaacctgg tgcgccgcac caaccgccag 361 ggtcgccggc ttgctcgccg caagaagcac cggcgcgtgc gcctgaaccg cctgttcgag 421 gagagcggcc tgatcaccga cttcaccaag atcagcatca acctgaaccc ctaccagctg 481 cgcgtgaagg gcctgaccga cgagctgagc aacgaggagc tgttcatcgc cctgaagaac 541 atggtgaagc accgcggcat cagctacctg gacgacgcca gcgacgacgg caacagcagc 601 gtgggcgact acgcccagat cgtgaaggag aacagcaagc agctggagac caagaccccc 661 ggccagatcc agctggagcg ctaccagacc tacggccagc tgcgcggcga cttcaccgtg 721 gagaaggacg gcaagaagca ccgcctgatc aacgtgttcc ccaccagcgc ctaccgcagc 781 gaggccctgc gcatcctgca gacccagcag gagttcaacc cccagatcac cgacgagttc 841 atcaaccgct acctggagat cctgaccggc aagcgcaagt actaccacgg //