LOCUS       Exported                 682 bp ds-DNA     linear   SYN 24-MAY-2021
DEFINITION  Recombine floxed alleles in any cell.
ACCESSION   .
VERSION     .
KEYWORDS    FUG-T2A-cre
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Williams MR, DeSpenza T Jr, Li M, Gulledge AT, Luikart BW
  TITLE     Hyperactivity of newborn Pten knock-out neurons results from 
            increased excitatory synaptic drive.
  JOURNAL   J Neurosci. 2015 Jan 21;35(3):943-59. doi: 
            10.1523/JNEUROSCI.3144-14.2015.
  PUBMED    25609613
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 24, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             16..69
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gacgagctgt acaaggaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
       61 cccggcccta gtacatccaa tttactgacc gtacaccaaa atttgcctgc attaccggtc
      121 gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga tcgccaggcg
      181 ttttctgagc atacctggaa aatgcttctg tccgtttgcc ggtcgtgggc ggcatggtgc
      241 aagttgaata accggaaatg ctttcccgca gaacctgaag atgttcgcga ttatcttcta
      301 tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg ccagctaaac
      361 atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt ttcactggtt
      421 atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca ggctctagcg
      481 ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga tcgctgccag
      541 gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg tatagccgaa
      601 attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat gttaatccat
      661 attggcagaa cgaaaacgct gg
//
