LOCUS       Exported                 888 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee SY, He M, Tucciarone
            J, Selimbeyoglu A, Berndt A, Grosenick L, Zalocusky KA, Bernstein H,
            Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, 
            Deisseroth K
  TITLE     Targeting cells with single vectors using multiple-feature Boolean 
            logic.
  JOURNAL   Nat Methods. 2014 Jul;11(7):763-72. doi: 10.1038/nmeth.2996. Epub 
            2014 Jun 8.
  PUBMED    24908100
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    194..227
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    278..311
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACCT). loxN sites are compatible with each 
                     other, but incompatible with loxP or lox2272 sites."
     primer_bind     424..445
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gtgccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc
      121 tgtgggacga catgacttaa ccagttaacc atgttctctg aacatgagaa gggctggcca
      181 ctctccagtc gacataactt cgtataggat actttatacg aagttatgca gaatggtagc
      241 tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata gtatacctta
      301 tacgaagtta tactagtcct cctgtactca ccttgccggt ggtgcagatg aacttcaggg
      361 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
      421 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
      481 tgctcacggc ggccgctggc acggctccgg cctcggcttc gtcttcgacg agagtctcga
      541 cctcgatctc cgttccgccg atgttcagtt tggtggtttt gcggatatct ccgtgaatca
      601 atatgtgctc gtggatcagg acgcgcaggt agtgtcccaa caacccccaa caatttttac
      661 tcatcagatc aataatcgtg tgacctacgg tggagccata gacgctcagg acgccaaaac
      721 cttcgggccc caaaatgaag agaattggga acatacccca gctcacgaaa aacagccatg
      781 ccatgccggt cacgacctgg cggcaccgac cctttggcac agtatgataa ccctcgatat
      841 atgctttggc ggcgtgaaaa aatgtgttcg cgccatagca caatccca
//
