LOCUS Exported 12416 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION 2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance. ACCESSION . VERSION . KEYWORDS phMYT1L-N174 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12416) AUTHORS Richner M, Victor MB, Liu Y, Abernathy D, Yoo AS TITLE MicroRNA-based conversion of human fibroblasts into striatal medium spiny neurons. JOURNAL Nat Protoc. 2015 Oct;10(10):1543-55. doi: 10.1038/nprot.2015.102. Epub 2015 Sep 17. PUBMED 26379228 REFERENCE 2 (bases 1 to 12416) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12416 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2221..2432 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" intron 2433..3371 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3328..3348 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" regulatory 3393..3402 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter 6994..7493 /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(7029..7052) /label=MSCV-rev /note="Murine stem cell virus, reverse primer" primer_bind 7426..7445 /label=mPGK-F /note="Mouse PGK promoter, forward primer" CDS 7520..8314 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7574..7593) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8184..8203 /label=Neo-F /note="Neomycin resistance gene, forward primer" misc_feature 8338..8926 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8391..8411) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8809..8820) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 9134..9767 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(9896..9912) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9896..9912) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9909..9931) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9920..9936 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9944..9974) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9989..10010 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10127..10144) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(10298..10883) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(10378..10397) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11054..11914) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11677..11696 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11915..12019) /gene="bla" /label=AmpR promoter polyA_signal 12067..12201 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(12104..12123) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 12158..12177 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggt gcccgtcagt 2221 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa 2281 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc 2341 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc 2401 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc 2461 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt 2521 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg 2581 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc 2641 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca 2701 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg 2761 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc 2821 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg 2881 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta 2941 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat 3001 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc 3061 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat 3121 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga 3181 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 3241 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 3301 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 3361 ttccatttca ggtgtcgtga ggatccgcgg ccgccaccat ggaggtggac accgaggaga 3421 agcggcatcg cacgcggtcc aaaggggttc gagttcccgt ggaaccagcc atacaagagc 3481 tgttcagctg tcccacccct ggctgtgacg gcagtggtca tgtcagtggc aaatatgcaa 3541 gacacagaag tgtatatggt tgtcccttgg cgaaaaaaag aaaaacacaa gataaacagc 3601 cccaggaacc tgctcctaaa cgaaagccat ttgccgtgaa agcagacagc tcctcagtgg 3661 atgagtgtga cgacagtgat gggactgagg acatggatga gaaggaggag gatgaggggg 3721 aggagtactc cgaggacaat gacgagccag gggatgagga cgaggaggac gaggaggggg 3781 accgggagga ggaggaggag atcgaggagg aggatgagga cgatgacgag gatggagaag 3841 atgtggagga tgaagaagag gaagaggagg aggaggagga ggaggaagag gaagaagaaa 3901 acgaagacca tcaaatgaat tgtcacaata ctcgaataat gcaagacaca gaaaaggatg 3961 ataacaataa tgacgaatat gacaattacg atgaactggt ggccaagtca ttgttaaacc 4021 tcggcaaaat cgctgaggat gcagcctacc gggccaggac tgagtcagaa atgaacagca 4081 atacctccaa tagtctggaa gacgatagtg acaaaaacga aaacctgggt cggaaaagtg 4141 agttgagttt agacttagac agtgatgttg ttagagaaac agtggactcc cttaaactat 4201 tagcccaagg acacggtgtt gtgctctcag aaaacatgaa tgacagaaat tatgcagaca 4261 gcatgtcgca gcaagacagt agaaatatga attacgtcat gttggggaag cccatgaaca 4321 atggactcat ggaaaagatg gtggaggaga gcgatgagga ggtgtgtctg agcagtctgg 4381 agtgtttgag gaatcagtgc ttcgacctgg ccaggaagct cagtgagacc aacccgcagg 4441 agaggaatcc gcagcagaac atgaacatcc gtcagcatgt ccggccagaa gaggacttcc 4501 caggaaggac gccggacaga aactactcgg acatgctgaa cctcatgcgg ctggaggagc 4561 agttgagccc ccggtcgaga gtgtttgcca gctgtgcgaa ggaggatggg tgtcatgagc 4621 gggacgacga taccacctct gtgaactcgg acaggtctga agaggtgttc gacatgacca 4681 aggggaacct gaccctgctg gagaaagcca tcgctttgga aacggaaaga gcaaaggcca 4741 tgagggagaa gatggccatg gaagctggga ggagggacaa tatgaggtca tatgaggacc 4801 agtctccgag acaacttccc ggggaggaca gaaagcctaa atccagtgac agccatgtca 4861 aaaagccata ctatgatccc tcaagaacag aaaagaaaga gagcaagtgt ccaacccccg 4921 ggtgtgatgg aaccggccac gtaactgggc tgtacccaca tcaccgcagc ctgtccggat 4981 gcccgcacaa agatagggtc cctccagaaa tccttgccat gcatgaaagt gtcctcaagt 5041 gccccactcc gggctgcacg gggcgcgggc atgtcaacag caacaggaac tcccaccgaa 5101 gcctctccgg atgcccgatc gctgcagcag agaaactggc caaggcacag gaaaagcacc 5161 agagctgcga cgtgtccaag tccagccagg cctcggaccg cgtgctcagg ccaatgtgct 5221 ttgtgaagca gctggagatt cctcagtatg gctacagaaa caatgtcccc acaactacgc 5281 cgcgttccaa cctggccaag gagctcgaga aatattccaa gacctcgttt gaatacaaca 5341 gttacgacaa ccatacttat ggcaagcgag ccatagctcc caaggtgcaa accagggata 5401 tatcccccaa aggatatgat gatgcgaagc ggtactgcaa ggaccccagc cccagcagca 5461 gcagcaccag cagctacgcg cccagcagca gcagcaacct gagctgcggc gggggcagca 5521 gcgccagcag cacgtgcagc aagagcagct tcgactacac gcacgacatg gaggcggccc 5581 acatggcggc caccgccatc ctcaacctgt ccacgcgctg ccgcgagatg ccgcagaacc 5641 tgagcaccaa gccgcaggac ctgtgcgcca cgcggaaccc tgacatggag gtggatgaga 5701 acgggaccct ggacctcagc atgaacaagc agaggccgcg ggacagctgc tgccccatcc 5761 tgacccctct ggagcccatg tccccccagc agcaggcagt gatgaacaac cggtgtttcc 5821 agctgggcga gggcgactgc tgggacttgc ccgtagacta caccaaaatg aaaccccgga 5881 ggatagacga ggacgagtcc aaagacatta ctccagaaga cttggaccca ttccaggagg 5941 ctctagaaga aagacggtat cccggggagg tgaccatccc aagtcccaaa cccaagtacc 6001 ctcagtgcaa ggagagcaaa aaggacttaa taactctgtc tggctgcccc ctggcggaca 6061 aaagcattcg aagtatgctg gccaccagct cccaagaact caagtgcccc acgcctggct 6121 gtgatggttc tggacatatc accggcaatt atgcttctca tcggagcctt tcaggttgcc 6181 caagagcaaa gaaaagtggt atcaggatag cacagagcaa agaagataaa gaagatcaag 6241 aacccatcag gtgtccggtc cccgggtgcg acggccaggg ccacatcact gggaagtacg 6301 cgtcccatcg cagcgcctcc gggtgcccct tggcggccaa gaggcagaaa gacgggtacc 6361 tgaatggctc ccagttctcc tggaagtcgg tcaagacgga aggcatgtcc tgccccacgc 6421 caggatgcga cggctcaggc cacgtcagcg gcagcttcct cacacaccgc agcttgtcag 6481 gatgcccgag agccacgtca gcgatgaaga aggcaaagct ttctggagag cagatgctga 6541 ccatcaaaca gcgggccagc aacggtatag aaaatgatga agaaatcaaa cagttagatg 6601 aagaaatcaa ggagctaaat gaatccaatt cccagatgga agccgatatg attaaactca 6661 gaactcagat taccacgatg gagagcaacc tgaagaccat cgaagaggag aacaaagtga 6721 ttgagcagca gaacgagtct ctcctccacg agctggcgaa cctgagccag tctctgatcc 6781 acagcctggc taacatccag ctgccgcaca tggatccaat caatgaacaa aattttgatg 6841 cttacgtgac tactttgacg gaaatgtata caaatcaaga tcgttatcag agtccagaaa 6901 ataaagccct actggaaaat ataaagcagg ctgtgagagg aattcaggtc tgaggccgcg 6961 ccggctctag atcgcgaacg cgtgaattct accgggtagg ggaggcgctt ttcccaaggc 7021 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 7081 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 7141 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 7201 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca 7261 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 7321 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 7381 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 7441 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca 7501 gcccaagctt cctaggacca tgattgaaca agatggattg cacgcaggtt ctccggccgc 7561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 7621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 7681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 7741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 7801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 7861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 7921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 7981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 8041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 8101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 8161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 8221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 8281 cgccttctat cgccttcttg acgagttctt ctgacccggg cggggcgcgt ctggaacaat 8341 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct 8401 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg 8461 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg 8521 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt 8581 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt 8641 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg 8701 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc 8761 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat 8821 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc 8881 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct 8941 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 9001 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggtttttcc agtcacacct 9061 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa 9121 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg 9181 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg 9241 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag 9301 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg 9361 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat 9421 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac 9481 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg 9541 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt 9601 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 9661 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 9721 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 9781 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc 9841 ttgacattgc tagcgtttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 9901 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 9961 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 10021 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 10081 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 10141 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 10201 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 10261 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 10321 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 10381 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 10441 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 10501 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 10561 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 10621 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 10681 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 10741 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 10801 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta 10861 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 10921 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 10981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 11041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 11101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 11161 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 11221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 11281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 11341 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 11401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 11461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 11521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 11581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 11641 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 11701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 11761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 11821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 11881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 11941 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 12001 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 12061 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 12121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 12181 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt 12241 cccaccccat accctattac cactgccaat tacctagtgg tttcatttac tctaaacctg 12301 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa 12361 cttagtagtt tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagt //