LOCUS Exported 646 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms. ACCESSION . VERSION . KEYWORDS pDD285 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 646) AUTHORS Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B TITLE Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. JOURNAL Genetics. 2015 Jun 3. pii: genetics.115.178335. PUBMED 26044593 REFERENCE 2 (bases 1 to 646) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..646 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(280..585) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind complement(382..401) /label=ccdB-fwd /note="ccdB gene, forward primer" ORIGIN 1 ttaccttgga tccgtacatg aaggaggtgg cgaggatgtc gaaggcgaat gggagtggtc 61 ctccctcgac ggccttgata cgcatggttt gggttccctc gtatggcttt ccctctccct 121 cggaggtgca cttgaagtgg tggttgttga cggttccctc catgtagagc ttcatatgca 181 tgttttcttt aatgagctcg gagaccatcg atgctcctga ggctcccgat gctccctagg 241 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag 301 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat 361 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat 421 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag 481 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag 541 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt 601 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctc //