LOCUS       Exported                 907 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  This plasmid can be used in the triple transfection method of AAV 
            vector production. It supplies the replication proteins from AAV2 
            and the 7M8 capsid protein..
ACCESSION   .
VERSION     .
KEYWORDS    7M8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Dalkara D, Byrne LC, Klimczak RR, Visel M, Yin L, Merigan WH, 
            Flannery JG, Schaffer DV
  TITLE     In vivo-directed evolution of a new adeno-associated virus for 
            therapeutic outer retinal gene delivery from the vitreous.
  JOURNAL   Sci Transl Med. 2013 Jun 12;5(189):189ra76. doi: 
            10.1126/scitranslmed.3005708.
  PUBMED    23761039
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(1..23)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     123..142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(819..840)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
       61 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      121 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      181 atgcgtaagg agaaaatacc gcatcaggcg attccaacat ccaataaatc atacaggcaa
      241 ggcaaagaat tagcaaaatt aagcaataaa gcctcagagc ataaagctaa atcggttgta
      301 ccaaaaacat tatgaccctg taatactttt gcgggagaag cctttatttc aacgcaagga
      361 taaaaatttt tagaaccctc atatatttta aatgcaatgc ctgagtaatg tgtaggtaaa
      421 gattcaaacg ggtgagaaag gccggagaca gtcaaatcac catcaatatg atattcaacc
      481 gttctagctg ataaattcat gccggagagg gtagctattt ttgagaggtc tctacaaagg
      541 ctatcaggtc attgcctgag agtctggagc aaacaagaga atcgatgaac ggtaatcgta
      601 aaactagcat gtcaatcata tgtaccccgg ttgataatca gaaaagcccc aaaaacagga
      661 agattgtata agcaaatatt taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
      721 atttttgtta aatcagctca ttttttaacc aatatgccga aatcggcaaa atcccttata
      781 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
      841 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
      901 cactacg
//
