LOCUS       Exported                5579 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses human ERRbeta2 splice variant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pSG5-ERRbeta2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Heckler MM, Riggins RB
  TITLE     ERRbeta Splice Variants Differentially Regulate Cell Cycle 
            Progression.
  JOURNAL   Cell Cycle. 2014 Dec 12:0.
  PUBMED    25496115
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        9..366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(32..52)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      217..352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     279..298
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     intron          395..967
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     975..994
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1022..1041
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1022..1040
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2575..2696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2612..2631)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2666..2685
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2730..2747)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2901..3489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2981..3000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3660..4520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4283..4302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4521..4625)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4693..4711
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4750..4772)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4872..4891
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4958..5413)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5095..5116)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5307..5326
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5543..5565
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5557..5574
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5558..5574
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1021 gtaatacgac tcactatagg gcgaattcat gagcagcgac gacaggcatc tgggctcctc
     1081 ctgcggctcc ttcatcaaga ccgagcccag cagcccctcc agcggaattg acgccctgag
     1141 ccatcatagc ccttccggct ccagcgatgc ctccggaggc ttcggactgg ctctgggcac
     1201 ccacgccaat ggactggata gcccccccat gtttgctggc gctggcctgg gaggaacacc
     1261 ttgcaggaag tcctacgagg actgcgcctc cggcatcatg gaggactccg ccatcaagtg
     1321 cgagtacatg ctgaacgcca tccccaagag actctgcctg gtgtgcggcg atatcgctag
     1381 cggataccac tatggcgtgg cctcctgcga ggcctgtaag gctttcttca agaggaccat
     1441 ccagggcaac atcgagtaca gctgccctgc tacaaacgag tgtgagatca ccaagagaag
     1501 gaggaagagc tgtcaggcgt gcaggtttat gaaatgcctg aaggtgggaa tgctgaagga
     1561 gggcgtgaga ctggacagag tgaggggcgg cagacagaag tacaagagga ggctcgattc
     1621 cgaatcctcc ccttatctga gcctgcagat ttcccccccc gccaagaaac ccctcaccaa
     1681 aatcgtcagc tacctgctgg tggctgaacc cgacaagctg tacgccatgc ctccccctgg
     1741 catgcctgaa ggcgacatca aggccctgac caccctgtgt gatctggctg acagggagct
     1801 ggtggtgatc attggatggg ccaagcatat ccccggcttt agctccctca gcctgggcga
     1861 ccagatgtcc ctgctccaga gcgcttggat ggagatcctg attctgggca tcgtgtacag
     1921 gtccctcccc tacgacgaca agctcgtgta cgccgaggac tacattatgg acgaggaaca
     1981 cagcaggctg gccggcctgc tggagctgta cagagccatc ctgcagctgg tcaggaggta
     2041 caagaagctc aaggtggaga aggaggagtt cgtgaccctc aaggccctgg ccctggccaa
     2101 ctccgattcc atgtacattg aggacctgga agccgtccag aagctgcagg acctgctgca
     2161 cgaggccctc caggattatg agctgtccca gaggcacgag gagccttgga gaaccggaaa
     2221 actgctcctg accctccccc tgctgaggca aaccgctgcc aaggccgtgc agcacttcta
     2281 cagcgtgaag ctgcagggaa aagtgcccat gcacaagctg ttcctggaga tgctggaagc
     2341 caaggcctgg gctagggccg actccctgca agagtggagg cccctggagc aggtgccttc
     2401 ccccctgcat agggccacca aaagacagca cgtgcacttc ctgacccctc tgcctcctcc
     2461 ccctagcgtc gcttgggtgg gaacagctca ggccggctac catctggagg tgttcctgcc
     2521 ccaaagggcc ggatggccta gagccgcctg aggatccaga tcttattaaa gcagaacttg
     2581 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2641 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2701 gtctggtcga ctctagactc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3061 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     3121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     3361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4501 cggaaatgtt gaatactcat actcttcttt tttcaatatt attgaagcat ttatcagggt
     4561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4681 ttaacctata aaaataggcg tatcacgagg cccctttcgt ctcgcgcgtt tcggtgatga
     4741 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4801 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4861 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4921 accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg
     4981 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     5041 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     5101 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     5161 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     5221 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     5281 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     5341 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5401 ctacagggcg cgtcgcgcca ttcgccattc aggctacgca actgttggga agggcgatcg
     5461 gtgcgggcct cttcgctatt acgccagctg gcgaaggggg gatgtgctgc aaggcgatta
     5521 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatt
//
