LOCUS Exported 1611 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone. ACCESSION . VERSION . KEYWORDS pFUGW-hGtACR2-EYFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1611) AUTHORS Govorunova EG, Sineshchekov OA, Janz R, Liu X, Spudich JL TITLE Natural light-gated anion channels: A family of microbial rhodopsins for advanced optogenetics. JOURNAL Science. 2015 Jun 25. pii: aaa7484. PUBMED 26113638 REFERENCE 2 (bases 1 to 1611) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1611 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 889..1605 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(931..952) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1192..1211) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1539..1560 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 ggatccatgg cctcccaggt cgtgtacggc gagtgggcca gcacccacac agagtgctac 61 aacatgagca ggatcgacag caccttcgtg agcctgctgc agctggtgtg ggccgtggtg 121 agcggatgcc agaccatctt catgatcagc agggccccca aggtgccctg ggagagcgtg 181 tacctgccct tcgtggagtc catcacatac gccctggcca gcaccggcaa tggcacactg 241 cagatgaggg acggcaggtt ctttccctgg tccaggatgg ccagctggct gtgcacatgc 301 cccattatgc tgggccagat ctccaacatg gctctggtga agtacaagag catccctctg 361 aaccccatcg cccaggccgc cagcattatc agggtggtga tgggcattac cgctaccatc 421 tcccccgccg agtacatgaa gtggctgttc ttcttcttcg gcgctacctg cctggtcttc 481 gaatacagcg tggtgttcac catcttccag gtgggactgt acggcttcga gtccgtgggc 541 acccctctgg cccagaaggt ggtggtgagg atcaagatgc tgaggctgat cttcttcatc 601 gcctggacca tgttccccat cgtgtggctg atttccccca ccggcgtgtg cgtgatccac 661 gagaacgtga gcgccatcct gtatctgctc gccgacggcc tgtgcaagaa cacctacggc 721 gtgatcctgt ggtccaccgc ctggggagtg ctcgagggaa agtgggaccc ggcttgcctc 781 cctggccagg agaagcctga ggccgacgac cccttcggac tgaaccacga gaagaatgcc 841 ccccccaacg acgaggtcaa catcagaatg ttcggcaggg cggccgccgt gagcaagggc 901 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 961 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 1021 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc 1081 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc 1141 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 1201 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 1261 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 1321 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac 1381 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 1441 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag 1501 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 1561 accgccgccg ggatcactct cggcatggac gagctgtaca agtaagaatt c //