LOCUS Exported 610 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS AAV-FLEX-rev-ChR2(H134R)-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 610) AUTHORS Atasoy D, Aponte Y, Su HH, Sternson SM TITLE A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping. JOURNAL J Neurosci. 2008 Jul 9. 28(28):7025-30. PUBMED 18614669 REFERENCE 2 (bases 1 to 610) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..610 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(64..97) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(168..201) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." regulatory 219..228 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 ttttgtaatc cagaggttga ttatcgataa gcttgatatc gaattcgaac ctcttcgagg 61 gacataactt cgtataatgt atgctatacg aagttatggg tcgatggtga gatctggact 121 agagggtcga tggtgatgct tggatcaaaa taggaagaat cccaatgata acttcgtata 181 aagtatccta tacgaagtta tactagaatt cggtaccggc caccatggac tatggcggcg 241 ctttgtctgc cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt 301 ccgtcctggt ccctgaggat caatgttact gtgccggatg gattgaatct cgcggcacga 361 acggcgctca gaccgcgtca aatgtcctgc agtggcttgc agcaggattc agcattttgc 421 tgctgatgtt ctatgcctac caaacctgga aatctacatg cggctgggag gagatctatg 481 tgtgcgccat tgaaatggtt aaggtgattc tcgagttctt ttttgagttt aagaatccct 541 ctatgctcta ccttgccaca ggacaccggg tgcagtggct gcgctatgca gagtggctgc 601 tcacttgtcc //