LOCUS       Exported                 716 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  Can be used to generate AAV virus that will express EGFP from the 
            tetO promoter in a Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    AAV pTRE-FLEX-EGFP-WPRE-bGHpA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van
            der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, 
            Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, 
            Helmchen F, Empson RM, Knopfel T, Boyden ES, Reid RC, Carandini M, 
            Zeng H
  TITLE     Transgenic mice for intersectional targeting of neural sensors and 
            effectors with high specificity and performance.
  JOURNAL   Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022.
  PUBMED    25741722
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    46..79
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     protein_bind    90..123
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      195..204
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             201..716
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNI"
     primer_bind     complement(246..267)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(507..526)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttgatgat cttcgataac ttcgtatagg
       61 atactttata cgaagttatt gcgattcgga taacttcgta tagcatacat tatacgaagt
      121 tatcaaggcc ggccaggttg gcgcgccaac tagctagcta tcaaggttag tacgttccaa
      181 gcttggggga attcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      241 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      301 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      361 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
      421 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      481 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      541 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      601 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      661 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatc
//
