LOCUS Exported 781 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Blue light dependent photoswitching system. ACCESSION . VERSION . KEYWORDS pcDNA3.1_pMagFast2(3x)-iRFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 781) AUTHORS Kawano F, Suzuki H, Furuya A, Sato M TITLE Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. JOURNAL Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256. PUBMED 25708714 REFERENCE 2 (bases 1 to 781) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..781 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 36..55 /label=T7 /note="T7 promoter, forward primer" promoter 36..54 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 114..563 /codon_start=1 /product="""positive Magnet"" variant of an N-terminally truncated Vivid (VVD) blue light photoreceptor from Neurospora crassa (Kawano et al., 2015)" /label=pMagFast2 /note="rapidly heterodimerizes with nMagHigh1 upon blue light irradiation to form a complex with fast switch-off kinetics" /translation="HTLYAPGGYDIMGYLRQIRNRPNPQVELGPVDTSCALVLCDLKQK DTPVVYASEAFLYMTGYSNAEVLGRNCRFLQSPDGMVKPKSTRKYVDSNTINTMRKAID RNAEVQVEVVNFKKNGQRFVNFLTMIPVRDETGEYRYSMGFQCETE" ORIGIN 1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc 61 caagctggct agcgtttaaa cttaagctta agctggctag cgccgccacc atgcacactc 121 tttacgcccc tggaggatac gacattatgg gatatttgcg gcagattagg aaccgcccaa 181 accctcaggt cgaactgggg cctgtggaca cgtcatgtgc cctggtcctg tgcgatctga 241 agcaaaagga cactccggtg gtctacgcct cggaagcctt cttgtatatg accggataca 301 gcaatgcaga ggtgctcggc aggaactgca gattcctgca gtcccccgac gggatggtga 361 aaccaaagtc gactcgcaaa tatgtggact cgaacacgat caacaccatg cggaaggcca 421 tcgaccggaa cgccgaggtc caggtggagg tggtcaactt taagaagaac ggccagcggt 481 tcgtgaactt tctgaccatg attccggtcc gggatgaaac cggagagtac agatactcca 541 tgggattcca gtgcgaaacc gaaggttccg gcggctcggg cggatcaggg ggaagcggac 601 acacactcta cgccccggga ggctacgata tcatgggcta cctgaggcag atccggaaca 661 gaccgaaccc ccaagtggaa ctcggccccg tggacacttc ttgcgctctg gtgctgtgcg 721 acctgaagca gaaggatacc cccgtggtgt acgcatccga agcgttcctc tacatgaccg 781 g //