LOCUS       Exported                 826 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Blue light dependent photoswitching system.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3.1_iSH2-pMag(3x)-iRFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Kawano F, Suzuki H, Furuya A, Sato M
  TITLE     Engineered pairs of distinct photoswitches for optogenetic control 
            of cellular proteins.
  JOURNAL   Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256.
  PUBMED    25708714
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      90..99
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagcttg ccaccatggt gtccaaatac caacaggatc
      121 aagttgtcaa agaagataat attgaagctg tagggaaaaa attacatgaa tataacactc
      181 aatttcaaga aaaaagtcgg gaatatgata gattatatga agactatacc cgaacttccc
      241 aggaaatcca aatgaaaaga acagctattg aagcatttaa tgaaaccata aaaatatttg
      301 aagaacagtg ccaaacccag gagcggtaca gcaaagaata catagaaaag ttcaaacgtg
      361 aaggcaacga gacggaaatc caaaggatta tgcataatta tgaaaagtta aagtctcgaa
      421 tcagtgaaat tgtcgacagc agaaggagat tggaagagga cttgaagaag caggcggctg
      481 agtatcgtga gattgacaaa cgcatgaaca gcattaaacc agacctcatt cagctaagaa
      541 agacgagaga ccaatacttg atgtggttga ctcagaaagg tgttcggcag aagaaattga
      601 acgagtggct gggcaatgaa aacactgaag accaatattc gctggtagaa gatgatgagg
      661 atttgcccca ccacgatgag aagactggat ccggcggctc cggaggcagc ggcggttctg
      721 gaggttccgg tggcggctcc cacactcttt acgcccctgg aggatacgac attatgggat
      781 atttgcggca gattaggaac cgcccaaacc ctcaggtcga actggg
//
