LOCUS Exported 902 bp ds-DNA linear SYN 03-JUN-2021 DEFINITION Expression of hArl4D with C-term V5 and His6 tag in E. coli. ACCESSION . VERSION . KEYWORDS pET-DEST42-ARL4D-V5-His6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 902) TITLE Unpublished Kahn lab ARF plasmids REFERENCE 2 (bases 1 to 902) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 3, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..902 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(29..46) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS complement(56..97) /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 117..141 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(763..787) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind 828..852 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(852..871) /label=T7 /note="T7 promoter, forward primer" promoter complement(853..871) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg gtacgcgtag 61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa ttgatcacca 121 ctttgtacaa gaaagctggg tcccgtctct tcttgccacc ccgagctgcc ttcttcctct 181 tgaggatcat ctcatagagg cgctcaaggc cctgctgcag gcccagaccg tccacagcgc 241 tgcagccttg cacatgagtg agagtggcgg ctgctagctc tcggactgcc agcctcttct 301 ccacctcagc agcgctcagt gccccgggct ggtcctgctt gttggccagc accagcactg 361 gcacgccctg gttgtccgag gcccggctga ttcggtgcaa ctccaccttg gcttcctcca 421 gccgctcagc ctccgcagcg tccaccacaa acactagacc gtctgtccgg cgggtataag 481 agcgccacag tggtcgcagc ttctcctgcc ccccgacgtc ccacacttgg aaggtgatgc 541 cacgcgatcc cccgaggggc acccggatct tctcggtgtt gaagcctttg gtggggacac 601 tctggacaaa ctccttgaac ttgaggcggt aaaggaggga ggtctttcca gcagagtcca 661 gcccaatgac cacgacatgc agggcttgga agtggggcaa gaaggaggag gcagtgggcg 721 ccatctcagt caagtggttc cccatggttc tatctccttc taagcctgct tttttgtaca 781 aacttgtgat attccttctt aaagttaaac aaaattattt ctagagggga attgttatcc 841 gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct 901 ac //