LOCUS Exported 941 bp ds-DNA linear SYN 01-JUN-2021 DEFINITION Expression of hArl14 with C-term V5 and His6 tag in E. coli. ACCESSION . VERSION . KEYWORDS pET-DEST42-ARL14-V5-His6 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 941) TITLE Unpublished Kahn lab ARF plasmids REFERENCE 2 (bases 1 to 941) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 1, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..941 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(29..46) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS complement(56..97) /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 117..141 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(736..760) /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind 801..825 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(825..844) /label=T7 /note="T7 promoter, forward primer" promoter complement(826..844) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 ggctttgtta gcagccggat caaactcaat ggtgatggtg atgatgaccg gtacgcgtag 61 aatcgagacc gaggagaggg ttagggatag gcttaccttc aagcttcgaa ttgatcacca 121 ctttgtacaa gaaagctggg tcgttctgct tgaagaacgc caaagtgtct cctcttgatt 181 tcatgtggct cttcacaaat ccagttaatt tcctgaaccc ctgggccagc ccctcccctg 241 tgagggcaca gcagggttgc acataccagt tccggtcact gcaaagcttc ttcactttga 301 acattctggt gatgtcctca gcagtcagag ctccaggcat gtcttgtttg ttggctaata 361 gaacaacagg cacattttta atgtgttcat tcttcaaaat gtgctcaaac tgtctctgag 421 actcttccag tcgctgtttg tctgtactgt ccacaacata caccagccca tcggtgttct 481 cacagtaaca gccccaaaca gttctcattt tttcctgtcc tccaacatcc cagactgtga 541 gtgaaaaatt cctttccaac tcgatcattt ccacattgaa acctattgta gggatggtgg 601 taatatcctt agcaagcttt aatttataaa ggagagtaga cttcccagct gagtcaagtc 661 ccaaaagaag aacttgggct tgtttggttt gcggattttt agaacccagc gaacccatgg 721 ttctatctcc ttctaagcct gcttttttgt acaaacttgt gatattcctt cttaaagtta 781 aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat agtgagtcgt 841 attaatttcg cgggaatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc 901 accggcgcca caggtgcggt tgctggcgcc tatatcgccg a //