LOCUS       Exported                 880 bp ds-DNA     linear   SYN 08-JUN-2021
DEFINITION  hArf3 with stop codon in Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    pDONR221-ARF3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 880)
  TITLE     Unpublished Kahn lab ARF plasmids
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    13..112
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(680..779)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(795..812)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(795..811)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(804..826)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcagctggat ggcaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
       61 attgataagc aatgctttct tataatgcca actttgtaca agaaagctgg gtcctatcac
      121 ttcttgtttt tgagctgatt ggccagccag tccaggcctt cgtacagccc gtccccgctg
      181 gtggcacagg tggcctgaat gtaccagtta cggtgacgaa gggaatgcag gcccagcttg
      241 tctgtgatct cagcagcgtt catagcatta ggcagatcct gtttgtttgc aaagacaagg
      301 agtacagcat cccggagctc gtcctccgcc agcattctca tcagctcttc ccgggcctca
      361 tttactcgct cccgatcatt gctgtcgacc acaaatatca acccttgggt gttctggaag
      421 tagtgtctcc agaggggtcg aatcttgtcc tggccaccca catcccacac tgtaaagctg
      481 atgttcttat actccactgt ctccacattg aacccaatgg tagggatggt ggtgacgatc
      541 tcccccagtt tcagcttgta taggatggtg gtctttcctg cggcatccag gcccaccatc
      601 aggatgcgca tctccttctt cccaatcagg ctcttgagaa ggtttccaaa gatattgccc
      661 atggttctat ctccttctaa gcctgctttt ttgtacaaag ttggcattat aaaaaagcat
      721 tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgg
      781 ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa catccgtgct
      841 agcgttaacg cgagagtagg gaactgccag gcatcaaata
//
