LOCUS       Exported                 914 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Mammalian expression of hSara with stop codon (native expression).
ACCESSION   .
VERSION     .
KEYWORDS    pDEST47-SARA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 914)
  TITLE     Unpublished Kahn lab ARF plasmids
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    58..82
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(701..725)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(758..776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(821..872)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(850..870)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttgc tagccattct agatcgaacc
       61 actttgtaca agaaagctgg gtcctattaa tcaatgtact gtgccatcca gcggaagcct
      121 tctccgtaac cttgtctttt gagcacacta cacatgaaaa cttctaaggg tcgggcattc
      181 agttctttca gagatatact cccctttcct gttgtctgac catataaacc aaacatctct
      241 cgcaacctct cttcactgat ggcttcaggt ctgtcgatct tattcccaag aatcagtata
      301 ggcacattag caatggtttc atctgtcatt agtgaatcaa gttcttcttt tgactctaac
      361 agcctttcgt ggtctgcaca atccaccaga aatacaatgc cattgatagc aggaaggtag
      421 tttttccaca ctcttcgagc ttgaacatgt ccacccagat caaaagttgt aaacgtcatg
      481 ccagcaatgg tcagttcttc ggaagtggga tgtaatgttg ggacatgttg tccaagtctg
      541 tcatctttta gcatgtgtag caatgttgtt tttcctgcat tatccaatcc aagaaatacc
      601 agtttaccag ttttcttata taatcctaaa aactgtagca cactgctgaa accactgtaa
      661 atccaatcaa atatgaagga catggttcta tctccttcta agcctgcttt tttgtacaaa
      721 cttgtttgat caagcttaac tagccagctt gggtctccct atagtgagtc gtattaattt
      781 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
      841 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      901 gaaagtcccg ttga
//
