LOCUS Exported 817 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION Targets HA-eGFP to mitochondrial matrix. ACCESSION . VERSION . KEYWORDS pCMV5-OCT-HA-eGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 817) TITLE Unpublished Kahn lab ARF plasmids REFERENCE 2 (bases 1 to 817) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..817 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 23..42 /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" CDS complement(67..783) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS complement(70..783) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(112..133) /label=EGFP-C /note="EGFP, forward primer" primer_bind 461..480 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 720..741 /label=EGFP-N /note="EGFP, reverse primer" CDS complement(790..816) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 ggcttcatga tgtccccata atttttggca gagggaaaaa gatctgctag caagcttctc 61 gagtcattac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc 121 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct 181 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta 241 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt 301 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc 361 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg 421 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc 481 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc 541 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt 601 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag 661 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac 721 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct 781 cacgaattca gcgtaatctg gaacatcgta tgggtag //