LOCUS Exported 757 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]. ACCESSION . VERSION . KEYWORDS pJ02B2Gm_AE SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 757) AUTHORS Iverson SV, Haddock TL, Beal J, Densmore DM TITLE CIDAR MoClo: Improved MoClo Assembly Standard and New E. coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology. JOURNAL ACS Synth Biol. 2016 Jan 15;5(1):99-103. doi: 10.1021/acssynbio.5b00124. Epub 2015 Nov 4. PUBMED 26479688 REFERENCE 2 (bases 1 to 757) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..757 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 15..23 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" misc_feature 152..173 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 176..219 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind complement(223..241) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(435..454) /label=pENTR-R /note="pENTR vectors, reverse primer" primer_bind complement(519..538) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" ORIGIN 1 cgcatcatta gaaaacctcc ttagcatgat taagatgttt cagtacgaaa attgctttca 61 ttgttgatct cctttttaag tgaacttggg cccagtagct agcacagtac ctaggactga 121 gctagctgtc aactccatgt cttccactag tctctagaag cggccgcgaa ttccagaaat 181 catccttagc gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat 241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaagtggc actcgagctg 301 atccttcaac tcagcaaaag ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct 361 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta 421 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctccag 481 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 541 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt 601 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 661 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 721 tgatgcatgg ttactcacca ctgcgatccc cgggaaa //