LOCUS Exported 10617 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Aurora A-GFP in mammalian cells. ACCESSION . VERSION . KEYWORDS pHR_dSV40-Aurora A-GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10617) AUTHORS Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD TITLE Regulation of mRNA translation during mitosis. JOURNAL Elife. 2015 Aug 25;4. doi: 10.7554/eLife.07957. PUBMED 26305499 REFERENCE 2 (bases 1 to 10617) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10617 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1..17) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2..18) /label=pBluescriptKS /note="For pBluescript vector" LTR 108..341 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(366..384) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(367..384) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(674..693) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind 1027..1049 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(1087..1105) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 1173..1277 /gene="bla" /label=AmpR promoter CDS 1278..2138 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1496..1515) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2309..2897 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2798..2817 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3051..3068 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(3138..3158) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3143..3472 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3323..3458 /label=SV40 ori /note="SV40 origin of replication" intron 4606..4671 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4801..4821 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5246..5380 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5283..5302) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5337..5356 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 5549..6182 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 6229..6354 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6846..7079 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7264..7308 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 7593..7710 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7773..7983 /label=SV40 promoter /note="SV40 early promoter" rep_origin 7842..7977 /label=SV40 ori /note="SV40 origin of replication" regulatory 7997..8006 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 8903..8914 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" regulatory 9242..9251 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9248..9967 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 9248..9964 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(9293..9314) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(9554..9573) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 9901..9922 /label=EGFP-C /note="EGFP, forward primer" misc_feature 10027..10615 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(10080..10100) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10498..10509) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta 61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg 361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg 421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag 481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc 541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc 601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac 901 tctcagtaca atctagctct gatgccgcat agttaagcca gccccgacac ccgccaacac 961 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga 1021 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac 1081 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 1141 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 1201 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 1261 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 1321 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 1381 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 1441 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 1501 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 1561 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 1621 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 1681 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 1741 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 1801 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 1861 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 1921 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 1981 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 2041 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 2101 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 2161 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 2221 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 2281 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 2341 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 2401 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 2461 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 2521 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 2581 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 2641 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 2701 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 2761 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 2821 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 2881 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 2941 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 3001 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 3061 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 3121 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg 3481 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag 3541 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct 3601 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg 3661 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac 3721 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc 3781 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac 3841 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc 3901 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc 3961 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc 4021 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca 4081 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg 4141 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa 4201 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc 4261 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc 4321 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc 4381 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg 4441 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg 4501 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt 4561 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt 4621 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc 4681 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc 4741 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct 4801 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt 4861 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag 4921 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt 4981 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg 5041 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg 5101 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac 5161 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 5221 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 5281 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 5341 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa 5401 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt 5461 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt 5521 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa 5581 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac 5641 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca 5701 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct 5761 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc 5821 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga 5881 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc 5941 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac 6001 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6061 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6121 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6181 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc 6241 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg 6301 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt 6361 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa 6421 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat 6481 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc 6541 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt 6601 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag 6661 caaaacaaaa gtaagaccac cgcacagcaa gcggccggtg atcttcagac ctggacgata 6721 tatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 6781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 6841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 6901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 6961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 7021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 7081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 7141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 7201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 7261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 7321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 7381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 7441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 7501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 7561 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg 7621 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta 7681 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca 7741 gtttggatcg ataagcttga tatcgaattc aggcaggcag aagtatgcaa agcatgcatc 7801 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 7861 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 7921 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 7981 gctacgcgtc agtcaggcca ccatggaccg atctaaagaa aactgcattt caggacctgt 8041 taaggctaca gctccagttg gaggtccaaa acgtgttctc gtgattcagc aatttccttg 8101 tcagaatcca ttacctgtaa atagtggcca ggctcagcgg gtcttgcgtc cttcaaattc 8161 ttcccagcgc gttcctttgc aagcacaaaa gcttgtctcc agtcacaagc cggttcagaa 8221 tcagaagcag aagcaattgc aggcaaccag tgtacctcat cctgtctcca ggccactgaa 8281 taacacccaa aagagcaagc agcccttgcc atcggcacct gaaaataatc ctgaggagga 8341 actggcatca aaacagaaaa atgaagaatc aaaaaagagg cagtgggctt tggaagactt 8401 tgaaattggt cgccctctgg gtaaaggaaa gtttggtaat gtttatttgg caagagaaaa 8461 gcaaagcaag tttattctgg ctctacgcgt gttatttaaa gctcagctgg agaaagccgg 8521 agtggagcat cagctcagaa gagaagtaga aatacagtcc caccttcggc atcctaatat 8581 tcttagactg tatggttatt tccatgatgc taccagagtc tacctaattc tggaatatgc 8641 accacttgga acagtttata gagaacttca gaaactttca aagtttgatg agcagagaac 8701 tgctacttat ataacagaat tggcaaatgc cctgtcttac tgtcattcga agagagttat 8761 tcatagagac attaagccag agaacttact tcttggatca gctggagagc ttaaaattgc 8821 agattttggg tggtcagtac atgctccatc ttccaggagg accactctct gtggcaccct 8881 ggactacctg ccccctgaaa tgattgaagg tcggatgcat gatgagaagg tggatctctg 8941 gagccttgga gttctttgct atgaattttt agttgggaag cctccttttg aggcaaacac 9001 ataccaagag acctacaaaa gaatatcacg ggttgaattc acattccctg actttgtaac 9061 agagggagcc agggacctca tttcaagact gttgaagcat aatcccagcc agaggccaat 9121 gctcagagaa gtacttgaac acccctggat cacagcaaat tcatcaaaac catcaaattg 9181 ccaaaacaaa gaatcagcta gcaaacagtc tggtggtgga tctggtgggg atccaccggt 9241 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 9301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 9361 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 9421 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 9481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 9541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 9601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 9661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 9721 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 9781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 9841 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 9901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 9961 caagtaagcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt 10021 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 10081 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 10141 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 10201 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 10261 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 10321 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 10381 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 10441 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 10501 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 10561 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcat //