LOCUS Exported 935 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation. ACCESSION . VERSION . KEYWORDS GPR153-Tango SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 935) AUTHORS Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, Sciaky N, Roth BL TITLE PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome. JOURNAL Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. Epub 2015 Apr 20. PUBMED 25895059 REFERENCE 2 (bases 1 to 935) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..935 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 47..66 /label=T7 /note="T7 promoter, forward primer" promoter 47..65 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 879..890 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact 61 atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc tcggatccac 121 tagtccagtg tggtggaatt ctgcagatat ccagcacagt ggcggccgcg ccaccatgaa 181 gacgatcatc gccctgagct acatcttctg cctggtattc gccgactaca aggacgatga 241 tgacgccagc atcgatatga gcgacgagag gagactgcct ggctctgcgg tggggtggct 301 ggtatgcggg gggctgtcac tccttgctaa cgcctggggg atcctcagcg tcggagcgaa 361 acagaagaag tggaagcctc ttgaattcct tctgtgtact cttgcagcaa cccacatgct 421 gaatgtagca gttcctattg ccacttattc cgtggttcag ttgagaaggc aaaggcccga 481 ctttgagtgg aatgagggct tgtgtaaggt ttttgtctcc acgttttaca cacttacact 541 ggccacctgt ttttcagtaa ctagcctgtc ataccaccgg atgtggatgg tatgctggcc 601 agtcaactat aggcttagta atgctaagaa acaggccgta cacactgtga tgggcatttg 661 gatggtaagt ttcatacttt ccgctttgcc agccgtgggc tggcatgata ccagtgaacg 721 gttctataca catgggtgtc ggttcattgt ggccgaaatt ggccttggat tcggcgtgtg 781 ctttttgctc ttggtcggcg gtagtgtggc catgggagtc atatgtaccg ctatcgccct 841 gttccagaca ctggccgtgc aggtggggag gcaggccgat cgaagggcgt ttactgtgcc 901 cactatagta gtggaagacg cacaggggaa aagaa //