LOCUS       Exported                 664 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    AAV-FLEX-rev-ChR2(H134R)-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Atasoy D, Aponte Y, Su HH, Sternson SM
  TITLE     A FLEX switch targets Channelrhodopsin-2 to multiple cell types for 
            imaging and long-range circuit mapping.
  JOURNAL   J Neurosci. 2008 Jul 9. 28(28):7025-30.
  PUBMED    18614669
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(72..105)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GGATACTT). lox2272 sites are compatible with each
                     other, but incompatible with loxP or loxN sites (Lee and 
                     Saito, 1988)."
     regulatory      123..132
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 tgggtcgatg gtgagatctg gactagaggg tcgatggtga tgcttggatc aaaataggaa
       61 gaatcccaat gataacttcg tataaagtat cctatacgaa gttatactag aattcggtac
      121 cggccaccat ggactatggc ggcgctttgt ctgccgtcgg acgcgaactt ttgttcgtta
      181 ctaatcctgt ggtggtgaac gggtccgtcc tggtccctga ggatcaatgt tactgtgccg
      241 gatggattga atctcgcggc acgaacggcg ctcagaccgc gtcaaatgtc ctgcagtggc
      301 ttgcagcagg attcagcatt ttgctgctga tgttctatgc ctaccaaacc tggaaatcta
      361 catgcggctg ggaggagatc tatgtgtgcg ccattgaaat ggttaaggtg attctcgagt
      421 tcttttttga gtttaagaat ccctctatgc tctaccttgc cacaggacac cgggtgcagt
      481 ggctgcgcta tgcagagtgg ctgctcactt gtcctgtcat ccttatccgc ctgagcaacc
      541 tcaccggcct gagcaacgac tacagcagga gaaccatggg actccttgtc tcagacatcg
      601 ggactatcgt gtggggggct accagcgcca tggcaaccgg ctatgttaaa gtcatcttct
      661 tttg
//
