LOCUS       Exported                8516 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  AAV vector expressing both SOD2 and mitochondrial targeted Catalase.
ACCESSION   .
VERSION     .
KEYWORDS    AAV-CMV-SOD2-2A-Catalase-WPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Xiong W, MacColl Garfinkel AE, Li Y, Benowitz LI, Cepko CL
  TITLE     NRF2 promotes neuronal survival in neurodegeneration and acute nerve
            damage.
  JOURNAL   J Clin Invest. 2015 Apr;125(4):1433-45. doi: 10.1172/JCI79735. Epub 
            2015 Mar 23.
  PUBMED    25798616
REFERENCE   2  (bases 1 to 8516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   4..133
                     /label=AAV2 ITR
     enhancer        212..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     746..766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     792..816
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          929..1404
                     /label=-beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             2139..2192
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3897..3917)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4315..4326)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4393..4410)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4399..4606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5299..5812
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5386..5405)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5596..5617
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6294..6313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6413..6435
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6473..6491)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6559..6663
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6664..7524
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6882..6901)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7695..8283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8184..8203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8437..8454
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtacatttat
      181 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag
      241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      601 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      721 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      781 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
      841 tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt
      901 accgagctcg gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc
      961 cccttctttt ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat
     1021 attgaccaaa tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat
     1081 atactttttt gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata
     1141 atgatacaat gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg
     1201 gttaaggcaa tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg
     1261 taagaggttt catattgcta atagcagcta caatccagct accattctgc ttttatttta
     1321 tggttgggat aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc
     1381 atacctctta tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat
     1441 cactttggca aagaattccg cgggcggccg ccatgttgtg tcgggcggcg tgcagcacgg
     1501 gcaggaggct gggccctgtg gccggtgccg cgggctcccg gcacaagcac agcctcccag
     1561 acctgcctta cgactatggc gcgctggagc cacacattaa cgcgcagatc atgcagctgc
     1621 accacagcaa gcaccacgcg gcctacgtga acaatctcaa cgccaccgag gagaagtacc
     1681 acgaggctct ggccaaggga gatgttacaa ctcaggtcgc tcttcagcct gcactgaagt
     1741 tcaatggtgg gggacatatt aatcacacca ttttctggac aaacctgagc cctaagggtg
     1801 gtggagaacc caaaggagag ttgctggagg ctatcaagcg tgactttggg tcttttgaga
     1861 agtttaagga gaagctgaca gccgtgtctg tgggagtcca aggttcaggc tggggctggc
     1921 ttggcttcaa taaggagcaa ggtcgcttac agattgctgc ctgctctaat caggacccat
     1981 tgcaaggaac aacaggcctt attccgctgc tggggattga cgtgtgggag cacgcttact
     2041 accttcagta taaaaacgtc agacctgact atctgaaagc tatttggaat gtaatcaact
     2101 gggagaatgt tactgaaaga tacacagctt gcaagaagga gggcagagga agtctgctaa
     2161 catgcggtga cgtcgaggag aatcctggcc caatgctgtt taatctgagg atcctgttaa
     2221 acaatgcagc ttttagaaat ggtcacaact tcatggttcg aaattttcgg tgtggacaac
     2281 cactacaatc ggacagtcgg gacccagcca gcgaccagat gaagcagtgg aaggagcagc
     2341 gggcctcgca gagacctgat gtcctgacca ccggaggcgg gaacccaata ggagataaac
     2401 ttaatatcat gaccgcgggg tcccgagggc ccctcctcgt tcaggatgtg gttttcactg
     2461 acgagatggc acactttgac agagagcgga ttcctgagag agtggtacac gcaaaaggag
     2521 caggtgcttt tggatacttt gaggtcaccc acgatatcac cagatactcc aaggcaaagg
     2581 tgtttgagca tattggaaag aggaccccta ttgccgttcg attctccaca gtcgctggag
     2641 agtcaggctc agctgacaca gttcgtgacc ctcgggggtt tgcagtgaaa ttttacactg
     2701 aagatggtaa ctgggatctt gtgggaaaca acacccctat tttcttcatc agggatgcca
     2761 tattgtttcc atcctttatc catagccaga agagaaaccc acagactcac ctgaaggatc
     2821 ctgacatggt ctgggacttc tggagtcttc gtcccgagtc tctccatcag gtttctttct
     2881 tgttcagtga ccgagggatt cccgatggtc accggcacat gaatggctat ggatcacaca
     2941 ccttcaagtt ggttaatgca gatggagagg cagtctattg caagttccat tacaagaccg
     3001 accagggcat caaaaacttg cctgttggag aggcaggaag gcttgctcag gaagatccgg
     3061 attatggcct ccgagatctt ttcaatgcca tcgccaatgg caattacccg tcctggacgt
     3121 tttacatcca ggtcatgact tttaaggagg cagaaacttt cccatttaat ccatttgatc
     3181 tgaccaaggt ttggcctcac aaggactacc ctcttatacc agttggcaaa ctggttttaa
     3241 acaaaaatcc agttaattac tttgctgaag ttgaacagat ggcttttgac ccaagcaata
     3301 tgccccctgg catcgagccc agccctgaca aaatgcttca gggccgcctt tttgcctacc
     3361 cggacactca ccgccaccgc ctgggaccca actatctgca gatacctgtg aactgtccct
     3421 accgcgctcg agtggccaac taccagcgtg atggccccat gtgcatgcat gacaaccagg
     3481 gtggtgcccc caactattac cccaacagct tcagcgcacc agagcagcag cgctcagccc
     3541 tggagcacag cgtccagtgc gctgtagatg tgaaacgctt caacagtgct aatgaagaca
     3601 atgtcactca ggtgcggaca ttctacacaa aggtgttgaa cgaggaggag aggaaacgcc
     3661 tgtgtgagaa cattgccggc cacctgaagg acgctcagct tttcattcag aagaaagcgg
     3721 tcaagaattt cactgacgtc caccctgact atggggcccg catccaggct cttctggaca
     3781 agtacaacgc tgagaagcct aagaacgcaa ttcacaccta cacgcaggcc taagcttgga
     3841 tccaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3901 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3961 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4021 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4081 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4141 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4201 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4261 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4321 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4381 cttcgagatc tgcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4561 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggactcg agtgctttat
     4621 ttgtgaaatt tgtgatacta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4681 taacaacaac aattgcattc attttattgt ttcaggttca gggggaggtg tgggaggttt
     4741 tttaaagggg gagggggtac gtagataagt agcatggcgg gttaatcatt aactacaagg
     4801 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     4861 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     4921 cgcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4981 agcctgaatg gcgaatggcg attctgttgc aatggctggc ggtaatattg ttctggatat
     5041 taccagcaag gccgatagtt tgagttcttc tactcaggca agtgatgtta ttactaatca
     5101 aagaagtatt gcgacaacgg ttaatttgcg tgatggacag actcttttac tcggtggcct
     5161 cactgattat aaaaacactt ctcaggattc tggcgtaccg ttcctgtcta aaatcccttt
     5221 aatcggcctc ctgtttagct cccgctctga ttctaacgag gaaagcacgt tatacgtgct
     5281 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5341 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5401 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5461 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5521 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5581 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5641 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5701 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttaaatat
     5761 ttgcttatac aatcttcctg tttttggggc ttttctgatt atcaaccggg gtacatatga
     5821 ttgacatgct agttttacga ttaccgttca tcgattctct tgtttgctcc agactctcag
     5881 gcaatgacct gatagccttt gtagagacct ctcaaaaata gctaccctct ccggcatgaa
     5941 tttatcagct agaacggttg aatatcatat tgatggtgat ttgactgtct ccggcctttc
     6001 tcacccgttt gaatctttac ctacacatta ctcaggcatt gcatttaaaa tatatgaggg
     6061 ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag tattacaggg
     6121 tcataatgtt tttggtacaa ccgatttagc tttatgctct gaggctttat tgcttaattt
     6181 tgctaattct ttgccttgcc tgtatgattt attggatgtt ggaatgccct gatgcggtat
     6241 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     6301 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     6361 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     6421 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     6481 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6541 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6601 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6661 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6721 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6781 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6841 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6901 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6961 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7021 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7081 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7141 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7201 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7261 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7321 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7381 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7441 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7501 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7561 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7801 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     7861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8341 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8401 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8461 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatg
//
