LOCUS Exported 11260 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells. ACCESSION . VERSION . KEYWORDS pmPB3-VSV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11260) AUTHORS Maier V, Jolicoeur C, Rayburn H, Takegahara N, Kumanogoh A, Kikutani H, Tessier-Lavigne M, Wurst W, Friedel RH TITLE Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development. JOURNAL Mol Cell Neurosci. 2011 Feb;46(2):419-31. doi: 10.1016/j.mcn.2010.11.005. Epub 2010 Nov 29. PUBMED 21122816 REFERENCE 2 (bases 1 to 11260) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11260 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1095..1127 /codon_start=1 /product="epitope tag from vesicular stomatitis virus G protein" /label=VSV-G tag /translation="YTDIEMNRLGK" promoter complement(6812..6830) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(6813..6830) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(6850..6867) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6856..7080 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7126..7554 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7213..7232) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7423..7444 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7563..7583) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7568..7898 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7749..7884 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7811..7830 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7965..8759 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(8019..8038) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8629..8648 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 8933..9054 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8970..8989) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9024..9043 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(9103..9119) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9103..9119) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9116..9138) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9127..9143 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9151..9181) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9196..9217 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9334..9351) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9505..10093) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9585..9604) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10264..11124) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10887..10906 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11125..11229) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcatgc 901 ctgcaggtca gcttgatttc acccaggcat ctggtccaga gggatccgcc agcccgtcca 961 gcagcaccat gtgggtgacc aaactcctgc cagccctgct gctgcagcat gtcctcctgc 1021 atctcctcct gctccccatc gccatcccct atgcagaggg acaaaggaaa agaagaaata 1081 caattcatga attctataca gacatagaga tgaaccgact tggaaagggc ggagtcgacc 1141 atcgcttctc tgtaccaaat accagcttca accacctggt gctggcacca gatcagggca 1201 aactctacgt gggtgcagtg aatcacctct ttcagctcag ccctgagctg aagatggagt 1261 ctgtggctgt cactggccct gtcattgaca gtcctgattg tgtgcctttc cgtgacctgg 1321 ctgagtgccc acaggcccag ctcactgaca atgccaacca gctcttgttg gtgagcagca 1381 ggacacagga actggtggct tgtgggcaag tgaagcaggg tgtatgtgag aagcggcgcc 1441 ttggggatgt gactcaagtg ttgtaccagg ctgaggaccc tggtgatggg cagtttgtgg 1501 ctgccaatac cctgggagtg accacagtag gcctagtggt gcccttgcca ggccgagacc 1561 tcctgctagt ggccagaggc ctggcaggca agctgtcagc aggagtgcca cccctaactg 1621 tgcgccagct ggctgggcca cagcctttct ccagtgaagg actgggaaga cttgtggtcg 1681 gtgacttttc agactacaac aatagctatg taggagcttt ttctgatgct cactctgcct 1741 actttgtgtt ccgtcgccgt ggggctcggg cccagactga gtatcgctcc tatgtagctc 1801 gtgtctgcct cagagatgtc aacctttatt cctatgtgga aatgccactt acctgccatg 1861 gccagggcct tatccaggct gccttcctta ctccagacac tttgctaggt gcattttcag 1921 caggcacaag tcaggcacag gcggccctgt gtgcctttcc cttagctgac ctggacagga 1981 gtatggaaca agccaggcga ctttgctata ctactggtgg tcagggtcct agcggcatgg 2041 aagaagccac tgtggagtat ggtgtcacat cccgttgcgt cactttgcct cctgactccc 2101 ccgagtcata cccctgtggt gatgaacaca cccccagccc catcgcaggc cgccaacccc 2161 tggaggccca gcctctgctg caactgggac agtccatcag tgcagtggct gctctccaga 2221 cagatgggca cacgatagcc ttcctgggag acacccaagg ccagctacat aaggtctttc 2281 taaacagctc ccatggccag gtataccatt cccagcaagt ggggccccca ggctcagcca 2341 tcagtccaga tctgctggta gacagcaatg gagaccacct ctatgtcttg actgcccagc 2401 aggtagaccg gatacttgtg gcagcctgtc ctcagtttcc taactgcact acctgtctcc 2461 aggccaggga cccactatgt ggttggtgca tccttcaggg aaggtgtact cggagaggcg 2521 agtgtggacg ggcagcccag ccaaaccact ggctgtggag ctacgaagac aaccactgcc 2581 cttacatcca gagcctgcta ccagcccaac acccccgtca ggaacagggt cagatcattc 2641 tgtctgttcc tcggctgccc accctggcca tggatgaata cttccactgt gcttttgggg 2701 gctacaacag tctagctcag gtggaagaac cccatgtggt ctgtaccacc cctcctcaag 2761 atcagatgcc acctaatcct ccaggctcag atcatgtcac cttgcccctg gccctaatgt 2821 ttgaggatgt ggtattgacc gccaccacat tctccttcta tgactgcagt gctgttcagg 2881 ccttggaagt ggctgccccg tgccgcgctt gtgtgagcag cctgtggcgg tgccactggt 2941 gcccccagag tagccactgt atatatggag agcactgccc agagggtgaa aaggctgtct 3001 acagtgccca ggaggtggac atcctggttc gtggcccaga ggcttgcccc caggttgagg 3061 gtctagcaag tccccagttg gtgcctgtgg gttgggagag ccatgtgacc ctgcacattc 3121 agaaccttca ctatttccaa ggtctgcctg ccttgtacca ctgctggcta gagctgcctg 3181 gaaaactgca aaagctacct gcctccttag aggagacatc cagggactct ggcctcattc 3241 actgccaggc ccagcagttc tacccctcta tgtctcagtg ggagctccca gtgcccatct 3301 atgtaaccag gggtgagatc cagcggctgg acaatgctgg tgatcttcat gtgactctat 3361 atgactgtgc catgggtcac cctgactgca gccactgcca ggcggccaat gggagcctga 3421 gctgtttgtg gtgtggtgat ggccagcctg cctgccgcta tgggcctttg tgcccaccag 3481 gtgctgtgga acagctatgc cctataccca gcattgatgt gattgaaccc ctgactggtc 3541 ccccagaagg tggcttggcc atcaccatcc tgggttccaa cctgggccag gcctttaatg 3601 atgtgcgaaa tgctgtaact gtggctggcc agccctgcaa ccctgaccct tctctctacc 3661 gcatctctgc tcggattgta tgtgtgacat ctcctgctcc caatggcact gctgggccag 3721 tccaagtggc cattaagagt cgtcctcctg gcatctcaac ccagaacttc acctaccagg 3781 accctgtcct gctgagcctg aatcctcagt ggggccccca ggcagggggc acccagctca 3841 ccatccatgg gcagtatctc cagacaggag gcaacatcag tgtctttgtg ggtgaccaac 3901 cttgccctat ccaggagcct gtgtgtcctg aagccatcat atgccacacc atgccccaga 3961 ctgagccagg agaagcagtg gtcctcatag tctttgggca tgttgaacgc aaactgctca 4021 ccaccccttt tcgatacaca gccaatcccc aactggtgga agcagagcct agtgtcagct 4081 tccggggagg tggacgagtg attcgagtca ggggtacagg cttagacgtt gtgtggcagc 4141 ccttgctgtc tgtgtggctg gaggatgagc ccaaagtaaa ggccttgggg gttcaggccc 4201 aggatgcgaa cccaaggagg agctgtggtg cccctgctgc agatcctcag gcctgtatcc 4261 atcttgagag tggcttacta cagtgttcca cgctctgctc tgtcaactca tccagcctcc 4321 tcctgtgcca cagtccagca gtgccagatg gggccctccc aaagcgagtc ttctttgccc 4381 tagacaacat gcaagtagac tttgccagtg ctagtggggg ccagggattc ctataccagc 4441 ctaaccctcg cctggctcca cttagtcatg aggggattac tcacccctac cacctcaagc 4501 caggccatgt cctagatgtg gagggcgagg gcctcaatct ggggatcagc aaggaggagg 4561 tgcaggtgca tattggagat ggagagtgcc tggtgaagac actgacgctt actcacttgt 4621 actgtgagcc accaccccaa gcccctcaac ccaccaatgg ttcaggcacc ctgccgcagt 4681 ttgtggtgca gatgggcaac ctgcgcctgg ctctaggccc tgtccagtac gaagcagagt 4741 ccatgatgtc cacctttcct gtggaggccc agttgggcct gggcatgggt gctgccgtgc 4801 tgattgctgc ggtgctcctc ctcaccctca tgtacaggca caagagcaag aaggccttgc 4861 gagactatca gaaggtgctg gtgcagttgg agaacctgga gactggtgtg ggtgaccagt 4921 gccgcaagga gttcacagac ttgatgactg agatgacaga cctcaccagt gacctagagg 4981 ccagtgggat tcccttcctg gactaccgaa catatgctga gcgtgccttc ttccccggcc 5041 atgttggctg cccactgcag ccagggctcg aggggcttgg ggaagagggt cgcagtgtca 5101 ccgtgcgcca gggtctcaca caactctcca atttgctcaa cagcaagctc ttccttctca 5161 cactcatcca caccttagag gagcagccaa gcttctccca gcgggaccgc tgccatgtgg 5221 cttcactact ttccctggct ctgcacagca agcttgagta tttgaccgac atcatgagaa 5281 ctctgcttgg tgacctagcg gcccattatg tacacaagaa ccccaagctc atgttacgca 5341 ggacggagac catggtagag aagctgctca ccaactggct atctatctgt ctctacacct 5401 tcttgaagga agtggctggc gagccactgt acatgctttt ccgagccatc aagtaccagg 5461 tggacaaggg ccctgtggac gctgtgacag gcaaggcaaa acgaactctg aatgacagcc 5521 acctgctacg agaggatgtg gagttccagc ccctgaccct gatggcactg gtgggaccag 5581 aggctgatag ggcagcaggg aacagtggag tgcatcgagt gccagccagg gtgcttgata 5641 cagacaccat cacccaagtc aaggagaagg tgttggacca aatctacaag ggaaccccct 5701 tctcccagag gccttcagtg cactccctag atcttgagtg gcgctcaggc ctagctggtc 5761 acctaaccct gtcagatgag gacttgacct ctgtgactca aaaccactgg aagagactca 5821 acaccctaca acactataag gtcccagatg gagcaacagt ggtactcatt cctcaggtgc 5881 acaatggtgg cactgtttcc cagagtctgg gccagactgg ctgcccttct ggagagaaca 5941 ctcccatgct ggaagatggt gaggaaggtg gagttcggct ctggcacctg gtaaaggcca 6001 ctgaggaggc agaaggggcc aaagtgcggc gcagcagcct ccgagatcga gaacgagaac 6061 ggtcacgagc caaggccatt cctgaaatct atcttactcg cttactttca atgaagggca 6121 ccctgcagaa gtttgtggat gacaccttcc aggccattct tagcatgaat cggcctgtgc 6181 ctattgctgt taagtaccta tttgacttcc tggatgagct ggcagagaaa catggcattg 6241 aagatccaga gaccctgcac atttggaaga caaacagcct acttctgcga ttctgggtga 6301 atgtcttgaa gaacccacag ctcatctttg atgtacaagt gtcagacaac gaggatgcca 6361 tcttggctgt cattgcacag actttcatcg attcctgcat ggtctcggag cataaagtgg 6421 gccgggattc cccagtgaac aaactgctct atgcccggga gatccctcgc tacaagcaga 6481 tggtggagaa gtactatgcg gacattcgcc agagctctcc agcaagctat caagagatga 6541 actcagctct ggctgaactc tctgggaatt attcctctgc tccccactgc ctggaggctc 6601 ttcgagaact ttacaaccac atccacaggt attatgacca gattatcagt gccctggagg 6661 aggaccctgt ggcccagaag atgcagctgg cttgccgcct gcagcaggtg gctgccttgg 6721 tggaatacaa agtgactgac ctgtgaacta gggccacagt gattacctga gacctgcgcg 6781 gccgctcgag catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc 6841 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 6901 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 6961 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 7021 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 7081 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 7141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 7201 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 7261 cccgtcaagc tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc 7321 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 7381 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 7441 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgggga 7501 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 7561 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccaggca ggcagaagta 7621 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 7681 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 7741 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 7801 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 7861 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 7921 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 7981 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 8041 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 8101 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc 8161 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 8221 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 8281 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 8341 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 8401 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 8461 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg 8521 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 8581 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 8641 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 8701 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag 8761 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt 8821 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg 8881 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt 8941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 9001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 9061 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt 9121 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 9181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 9241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 9301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 9361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 9421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 9481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 9541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 9601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 9661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct 9721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 9781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 9841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 9901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 9961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 10021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 10081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 10141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 10201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 10261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 10321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 10381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 10441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 10501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 10561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 10621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 10681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 10741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 10801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 10861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 10921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 10981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 11041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 11101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 11161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 11221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //