LOCUS Exported 828 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION mammalian expression of ER localized oxBFP. ACCESSION . VERSION . KEYWORDS ERoxBFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 828) AUTHORS Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL TITLE A palette of fluorescent proteins optimized for diverse cellular environments. JOURNAL Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. PUBMED 26158227 REFERENCE 2 (bases 1 to 828) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..828 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..93 /codon_start=1 /product="signal sequence from bovine prolactin" /label=prolactin signal sequence /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVST" regulatory 103..112 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 109..828 /codon_start=1 /product="enhanced blue variant of GFP (Ai et al., 2007)" /label=EBFP2 /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL EFRTAAGITLGMDELYK" primer_bind complement(154..175) /label=EGFP-N /note="EGFP, reverse primer" ORIGIN 1 atggacagca aaggttcgtc gcagaaaggg tcccgcctgc tcctgctgct ggtggtgtca 61 aatctactct tgtgccaggg tgtggtctcc accggaccgg tcgccaccat ggtgagcaag 121 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 181 ggccacaagt tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg caagctgacc 241 ctgaagttca tcagtactac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 301 ctgagccacg gcgtgcaggt gttcgcccgc taccccgacc acatgaagca gcacgacttc 361 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 421 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 481 gagctgaagg gcgtcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 541 aacttcaaca gccacaacat ctatatcatg gccgtcaagc agaagaacgg catcaaggcc 601 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag 661 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acagccacta cctgagcacc 721 cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 781 cgcaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaa //