LOCUS Exported 776 bp ds-DNA linear SYN 14-JUN-2021 DEFINITION Standard for cell-free expression benchmarking.. ACCESSION . VERSION . KEYWORDS pCellFree_G03 IFNG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 776) AUTHORS Gagoski D, Polinkovsky ME, Mureev S, Kunert A, Johnston W, Gambin Y, Alexandrov K TITLE Performance benchmarking of four cell-free protein expression systems. JOURNAL Biotechnol Bioeng. 2015 Aug 24. doi: 10.1002/bit.25814. PUBMED 26301602 REFERENCE 2 (bases 1 to 776) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 14, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..776 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(12..35) /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" primer_bind complement(178..197) /label=T7 /note="T7 promoter, forward primer" promoter complement(179..197) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 229..333 /gene="bla" /label=AmpR promoter primer_bind complement(552..571) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 ttgcttgtgc cgtggtgatg gtggtgatgg tggtgaccag aggtgcccat ggtttcactt 61 acgtgtttaa tgtctttaca gactttatac attactgtca tggttaaata aaataaaata 121 aaataaaata aaataaaata aaataaaata aaataaaata aaataaactt aagatgtccc 181 tatagtgagt cgtattagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 241 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 301 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 361 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 421 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 481 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 541 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 601 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 661 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 721 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgc //